Super-resolution two-photon microscopy via scanning patterned illumination

Ben E. Urban, Ji Yi, Siyu Chen, Biqin Dong, Yongling Zhu, Steven H DeVries, Vadim Backman, Hao F Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We developed two-photon scanning patterned illumination microscopy (2P-SPIM) for super-resolution two-photon imaging. Our approach used a traditional two-photon microscopy setup with temporally modulated excitation to create patterned illumination fields. Combing nine different illuminations and structured illumination reconstruction, super-resolution imaging was achieved in two-photon microscopy. Using 2P-SPIM we achieved a lateral resolution of 141 nm, which represents an improvement by a factor of 1.9 over the corresponding diffraction limit. We further demonstrated super-resolution cellular imaging by 2P-SPIM to image actin cytoskeleton in mammalian cells and three-dimensional imaging in highly scattering retinal tissue.

Original languageEnglish (US)
Article number042703
JournalPhysical Review E - Statistical, Nonlinear, and Soft Matter Physics
Volume91
Issue number4
DOIs
StatePublished - Apr 7 2015

Fingerprint

Super-resolution
Microscopy
Scanning
Illumination
Photon
illumination
microscopy
scanning
Imaging
photons
Three-dimensional Imaging
Cytoskeleton
Actin
Diffraction
Lateral
Excitation
Scattering
Cell
scattering
diffraction

ASJC Scopus subject areas

  • Statistical and Nonlinear Physics
  • Statistics and Probability
  • Condensed Matter Physics

Cite this

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abstract = "We developed two-photon scanning patterned illumination microscopy (2P-SPIM) for super-resolution two-photon imaging. Our approach used a traditional two-photon microscopy setup with temporally modulated excitation to create patterned illumination fields. Combing nine different illuminations and structured illumination reconstruction, super-resolution imaging was achieved in two-photon microscopy. Using 2P-SPIM we achieved a lateral resolution of 141 nm, which represents an improvement by a factor of 1.9 over the corresponding diffraction limit. We further demonstrated super-resolution cellular imaging by 2P-SPIM to image actin cytoskeleton in mammalian cells and three-dimensional imaging in highly scattering retinal tissue.",
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Super-resolution two-photon microscopy via scanning patterned illumination. / Urban, Ben E.; Yi, Ji; Chen, Siyu; Dong, Biqin; Zhu, Yongling; DeVries, Steven H; Backman, Vadim; Zhang, Hao F.

In: Physical Review E - Statistical, Nonlinear, and Soft Matter Physics, Vol. 91, No. 4, 042703, 07.04.2015.

Research output: Contribution to journalArticle

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AU - Zhang, Hao F

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