Superinduction of the Dictyostelium discoideum cell surface cAMP receptor by pulses of cAMP

R. L. Chisholm, S. Hopkinson, H. F. Lodish

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Extracellular cAMP plays a crucial role in regulating the developmental program of Dictyostelium discoideum, functioning as a chemotactic agent, as well as a signal that regulates expression of developmentally expressed genes. These activities appear to be mediated by a cell-surface receptor for cAMP. We have studied the regulation of this receptor in cells developed in starved suspension cultures exposed to 50 nM pulses of cAMP every 6 min. cAMP-pulsed cells display roughly 10-fold higher cAMP receptor levels than cells that developed on filters or that were starved in suspension without cAMP pulses. Based on saturation binding analysis, the superinduced binding activity represents an increase in receptor number, while receptor affinity for cAMP is unaffected. Photoaffinity labeling of superinduced cells results in specific labeling of the same molecules that are labeled in starved cells. This increased cAMP binding activity was also detected in membrane preparations from cAMP-pulsed cells. These results provide evidence for an unusual mode of receptor regulation: autogenous induction of the receptor by its ligand.

Original languageEnglish (US)
Pages (from-to)1030-1034
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number4
DOIs
StatePublished - 1987

ASJC Scopus subject areas

  • General

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