Supramolecular delivery of photoactivatable fluorophores in developing embryos

Yang Zhang; Sicheng Tang; Lorenzo Sansalone; Ek Raj Thapaliya; James D. Baker; Françisco M. Raymo

doi:10.1117/12.2261721

Supramolecular delivery of photoactivatable fluorophores in developing embryos

Yang Zhang^*, Sicheng Tang, Lorenzo Sansalone, Ek Raj Thapaliya, James D. Baker, Françisco M. Raymo

^*Corresponding author for this work

Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The identification of noninvasive strategies to monitor dynamics within living organisms in real time is essential to elucidate the fundamental factors governing a diversity of biological processes. This study demonstrates that the supramolecular delivery of photoactivatable fluorophores in Drosophila melanogaster embryos allows the real-time tracking of translocating molecules. The designed photoactivatable fluorophores switch from an emissive reactant to an emissive product with spectrally-resolved fluorescence, under moderate blue-light irradiation conditions. These hydrophobic fluorescent probes can be encapsulated within supramolecular hosts and delivered to the cellular blastoderm of the embryos. Thus, the combination of supramolecular delivery and fluorescence photoactivation translates into a noninvasive method to monitor dynamics in vivo and can evolve into a general chemical tool to track motion in biological specimens.

Original language	English (US)
Title of host publication	Colloidal Nanoparticles for Biomedical Applications XII
Editors	Marek Osinski, Wolfgang J. Parak, Xing-Jie Liang
Publisher	SPIE
ISBN (Electronic)	9781510605978
DOIs	https://doi.org/10.1117/12.2261721
State	Published - 2017
Event	Colloidal Nanoparticles for Biomedical Applications XII 2017 - San Francisco, United States Duration: Jan 28 2017 → Jan 31 2017

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10078
ISSN (Print)	1605-7422

Conference

Conference	Colloidal Nanoparticles for Biomedical Applications XII 2017
Country/Territory	United States
City	San Francisco
Period	1/28/17 → 1/31/17

Funding

The National Science Foundation (CHE-1049860) is acknowledged for financial support.

Keywords

Drosophila Melanogaster
Photoactivation
fluorescence imaging
molecular switches
nanoparticles
self-assembly

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2261721

Cite this

Zhang, Y., Tang, S., Sansalone, L., Thapaliya, E. R., Baker, J. D., & Raymo, F. M. (2017). Supramolecular delivery of photoactivatable fluorophores in developing embryos. In M. Osinski, W. J. Parak, & X.-J. Liang (Eds.), Colloidal Nanoparticles for Biomedical Applications XII Article 100781A (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10078). SPIE. https://doi.org/10.1117/12.2261721

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title = "Supramolecular delivery of photoactivatable fluorophores in developing embryos",

abstract = "The identification of noninvasive strategies to monitor dynamics within living organisms in real time is essential to elucidate the fundamental factors governing a diversity of biological processes. This study demonstrates that the supramolecular delivery of photoactivatable fluorophores in Drosophila melanogaster embryos allows the real-time tracking of translocating molecules. The designed photoactivatable fluorophores switch from an emissive reactant to an emissive product with spectrally-resolved fluorescence, under moderate blue-light irradiation conditions. These hydrophobic fluorescent probes can be encapsulated within supramolecular hosts and delivered to the cellular blastoderm of the embryos. Thus, the combination of supramolecular delivery and fluorescence photoactivation translates into a noninvasive method to monitor dynamics in vivo and can evolve into a general chemical tool to track motion in biological specimens.",

keywords = "Drosophila Melanogaster, Photoactivation, fluorescence imaging, molecular switches, nanoparticles, self-assembly",

author = "Yang Zhang and Sicheng Tang and Lorenzo Sansalone and Thapaliya, {Ek Raj} and Baker, {James D.} and Raymo, {Fran{\c c}isco M.}",

note = "Publisher Copyright: {\textcopyright} 2017 SPIE.; Colloidal Nanoparticles for Biomedical Applications XII 2017 ; Conference date: 28-01-2017 Through 31-01-2017",

year = "2017",

doi = "10.1117/12.2261721",

language = "English (US)",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

editor = "Marek Osinski and Parak, {Wolfgang J.} and Xing-Jie Liang",

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}

Zhang, Y, Tang, S, Sansalone, L, Thapaliya, ER, Baker, JD & Raymo, FM 2017, Supramolecular delivery of photoactivatable fluorophores in developing embryos. in M Osinski, WJ Parak & X-J Liang (eds), Colloidal Nanoparticles for Biomedical Applications XII., 100781A, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10078, SPIE, Colloidal Nanoparticles for Biomedical Applications XII 2017, San Francisco, United States, 1/28/17. https://doi.org/10.1117/12.2261721

Supramolecular delivery of photoactivatable fluorophores in developing embryos. / Zhang, Yang; Tang, Sicheng; Sansalone, Lorenzo et al.
Colloidal Nanoparticles for Biomedical Applications XII. ed. / Marek Osinski; Wolfgang J. Parak; Xing-Jie Liang. SPIE, 2017. 100781A (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10078).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Supramolecular delivery of photoactivatable fluorophores in developing embryos

AU - Zhang, Yang

AU - Tang, Sicheng

AU - Sansalone, Lorenzo

AU - Thapaliya, Ek Raj

AU - Baker, James D.

AU - Raymo, Françisco M.

PY - 2017

Y1 - 2017

N2 - The identification of noninvasive strategies to monitor dynamics within living organisms in real time is essential to elucidate the fundamental factors governing a diversity of biological processes. This study demonstrates that the supramolecular delivery of photoactivatable fluorophores in Drosophila melanogaster embryos allows the real-time tracking of translocating molecules. The designed photoactivatable fluorophores switch from an emissive reactant to an emissive product with spectrally-resolved fluorescence, under moderate blue-light irradiation conditions. These hydrophobic fluorescent probes can be encapsulated within supramolecular hosts and delivered to the cellular blastoderm of the embryos. Thus, the combination of supramolecular delivery and fluorescence photoactivation translates into a noninvasive method to monitor dynamics in vivo and can evolve into a general chemical tool to track motion in biological specimens.

AB - The identification of noninvasive strategies to monitor dynamics within living organisms in real time is essential to elucidate the fundamental factors governing a diversity of biological processes. This study demonstrates that the supramolecular delivery of photoactivatable fluorophores in Drosophila melanogaster embryos allows the real-time tracking of translocating molecules. The designed photoactivatable fluorophores switch from an emissive reactant to an emissive product with spectrally-resolved fluorescence, under moderate blue-light irradiation conditions. These hydrophobic fluorescent probes can be encapsulated within supramolecular hosts and delivered to the cellular blastoderm of the embryos. Thus, the combination of supramolecular delivery and fluorescence photoactivation translates into a noninvasive method to monitor dynamics in vivo and can evolve into a general chemical tool to track motion in biological specimens.

KW - Drosophila Melanogaster

KW - Photoactivation

KW - fluorescence imaging

KW - molecular switches

KW - nanoparticles

KW - self-assembly

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T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Colloidal Nanoparticles for Biomedical Applications XII

A2 - Osinski, Marek

A2 - Parak, Wolfgang J.

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Y2 - 28 January 2017 through 31 January 2017

ER -

Supramolecular delivery of photoactivatable fluorophores in developing embryos

Abstract

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