Abstract
The identification of noninvasive strategies to monitor dynamics within living organisms in real time is essential to elucidate the fundamental factors governing a diversity of biological processes. This study demonstrates that the supramolecular delivery of photoactivatable fluorophores in Drosophila melanogaster embryos allows the real-time tracking of translocating molecules. The designed photoactivatable fluorophores switch from an emissive reactant to an emissive product with spectrally-resolved fluorescence, under moderate blue-light irradiation conditions. These hydrophobic fluorescent probes can be encapsulated within supramolecular hosts and delivered to the cellular blastoderm of the embryos. Thus, the combination of supramolecular delivery and fluorescence photoactivation translates into a noninvasive method to monitor dynamics in vivo and can evolve into a general chemical tool to track motion in biological specimens.
Original language | English (US) |
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Title of host publication | Colloidal Nanoparticles for Biomedical Applications XII |
Editors | Marek Osinski, Wolfgang J. Parak, Xing-Jie Liang |
Publisher | SPIE |
ISBN (Electronic) | 9781510605978 |
DOIs | |
State | Published - 2017 |
Event | Colloidal Nanoparticles for Biomedical Applications XII 2017 - San Francisco, United States Duration: Jan 28 2017 → Jan 31 2017 |
Publication series
Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
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Volume | 10078 |
ISSN (Print) | 1605-7422 |
Conference
Conference | Colloidal Nanoparticles for Biomedical Applications XII 2017 |
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Country/Territory | United States |
City | San Francisco |
Period | 1/28/17 → 1/31/17 |
Funding
The National Science Foundation (CHE-1049860) is acknowledged for financial support.
Keywords
- Drosophila Melanogaster
- Photoactivation
- fluorescence imaging
- molecular switches
- nanoparticles
- self-assembly
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging