Surface-enhanced Raman sensing: Glucose and anthrax

N. C. Shah*, O. Lyandres, C. R. Yonzon, X. Zhang, R. P. Van Duyne

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations


This chapter outlines the use of surface-enhanced Raman spectroscopy (SERS) in the development of biological sensors for the detection of anthrax and glucose. In both cases, Ag film over nanosphere (AgFON) surfaces were used as the sensing platform. The localized surface plasmon resonance (LSPR) of AgFON surfaces was tuned to maximize the SERS signal for near infrar-red (NIR) excitations. A harmless analog of Bacillus anthracis, namely Bacillus subtilis, was quantitatively detected using SERS on AgFON surfaces. Calcium dipicolinate, an important biomarker for bacillus spores, was successfully measured with a limit of detection well below the anthrax infectious dose of 10 4 spores in 11 minutes. For glucose detection, a mixed self-assembled monolayer (SAM) consisting of decanethiol (DT) and mercaptohexanol (MH) was immobilized on an AgFON surface to bring glucose within the zone of the localized electromagnetic field. Complete partitioning and departitioning of glucose was demonstrated. Furthermore, quantitative detection in vitro and in vivo was achieved.

Original languageEnglish (US)
Title of host publicationNew Approaches in Biomedical Spectroscopy
EditorsKatrin Kneipp, Harald Kneipp, Ricardo Aroca, Edeline Wentrup-Byrne
Number of pages16
StatePublished - 2007

Publication series

NameACS Symposium Series
ISSN (Print)0097-6156

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering


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