Surface organization and nanopatterning of collagen by dip-pen nanolithography

Donna L. Wilson, Raquel Martin, Seunghun Hong, Mark Cronin-Golomb, Chad A. Mirkin, David L. Kaplan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

287 Scopus citations


Collagen is a key fibrous protein in biological systems, characterized by a complex structural hierarchy as well as the ability to self-assemble into liquid crystalline mesophases. The structural features of collagen influence cellular responses and material properties, with importance for a wide range of biomaterials and tissue architectures. The mechanism by which fibrillar collagen structures form from liquid crystalline mesophases is not well characterized. We report positive printing of collagen and a collagen-like peptide down to 30-50-nm line widths, using the atomic force microscopy technique of dip-pen nanolithography. The method preserved the triple-helical structure and biological activity of collagen and even fostered the formation of characteristic higher levels of structural organization. The "direct-write" capability of biologically relevant molecules, while preserving their structure and functionality, provides tremendous flexibility in future biological device applications and in proteomics arrays, as well as a new strategy to study the important hierarchical assembly processes of biological systems.

Original languageEnglish (US)
Pages (from-to)13660-13664
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number24
StatePublished - Nov 20 2001

ASJC Scopus subject areas

  • General


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