Susceptibility of outer hair cells to cholesterol chelator 2-hydroxypropyl-β-cyclodextrine is prestin-dependent

Satoe Takahashi, Kazuaki Homma, Yingjie Zhou, Shinichi Nishimura, Chongwen Duan, Jessie Chen, Aisha Ahmad, Mary Ann Cheatham, Jing Zheng

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Niemann-Pick type C1 disease (NPC1) is a fatal genetic disorder caused by impaired intracellular cholesterol trafficking. Recent studies reported ototoxicity of 2-hydroxypropyl- β-cyclodextrin (HPβCD), a cholesterol chelator and the only promising treatment for NPC1. Because outer hair cells (OHCs) are the only cochlear cells affected by HPβCD, we investigated whether prestin, an OHC-specific motor protein, might be involved. Single, high-dose administration of HPβCD resulted in OHC death in prestin wildtype (WT) mice whereas OHCs were largely spared in prestin knockout (KO) mice in the basal region, implicating prestin's involvement in ototoxicity of HPβCD. We found that prestin can interact with cholesterol in vitro, suggesting that HPβCD-induced ototoxicity may involve disruption of this interaction. Time-lapse analysis revealed that OHCs isolated from WT animals rapidly deteriorated upon HPβCD treatment while those from prestin-KOs tolerated the same regimen. These results suggest that a prestin-dependent mechanism contributes to HPβCD ototoxicity.

Original languageEnglish (US)
Article number21973
JournalScientific Reports
Volume6
DOIs
StatePublished - Feb 23 2016

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Outer Auditory Hair Cells
Cyclodextrins
Chelating Agents
Cholesterol
Type C Niemann-Pick Disease
Inborn Genetic Diseases
Knockout Mice
Cell Death

ASJC Scopus subject areas

  • General

Cite this

Takahashi, Satoe ; Homma, Kazuaki ; Zhou, Yingjie ; Nishimura, Shinichi ; Duan, Chongwen ; Chen, Jessie ; Ahmad, Aisha ; Cheatham, Mary Ann ; Zheng, Jing. / Susceptibility of outer hair cells to cholesterol chelator 2-hydroxypropyl-β-cyclodextrine is prestin-dependent. In: Scientific Reports. 2016 ; Vol. 6.
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abstract = "Niemann-Pick type C1 disease (NPC1) is a fatal genetic disorder caused by impaired intracellular cholesterol trafficking. Recent studies reported ototoxicity of 2-hydroxypropyl- β-cyclodextrin (HPβCD), a cholesterol chelator and the only promising treatment for NPC1. Because outer hair cells (OHCs) are the only cochlear cells affected by HPβCD, we investigated whether prestin, an OHC-specific motor protein, might be involved. Single, high-dose administration of HPβCD resulted in OHC death in prestin wildtype (WT) mice whereas OHCs were largely spared in prestin knockout (KO) mice in the basal region, implicating prestin's involvement in ototoxicity of HPβCD. We found that prestin can interact with cholesterol in vitro, suggesting that HPβCD-induced ototoxicity may involve disruption of this interaction. Time-lapse analysis revealed that OHCs isolated from WT animals rapidly deteriorated upon HPβCD treatment while those from prestin-KOs tolerated the same regimen. These results suggest that a prestin-dependent mechanism contributes to HPβCD ototoxicity.",
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Susceptibility of outer hair cells to cholesterol chelator 2-hydroxypropyl-β-cyclodextrine is prestin-dependent. / Takahashi, Satoe; Homma, Kazuaki; Zhou, Yingjie; Nishimura, Shinichi; Duan, Chongwen; Chen, Jessie; Ahmad, Aisha; Cheatham, Mary Ann; Zheng, Jing.

In: Scientific Reports, Vol. 6, 21973, 23.02.2016.

Research output: Contribution to journalArticle

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AU - Takahashi, Satoe

AU - Homma, Kazuaki

AU - Zhou, Yingjie

AU - Nishimura, Shinichi

AU - Duan, Chongwen

AU - Chen, Jessie

AU - Ahmad, Aisha

AU - Cheatham, Mary Ann

AU - Zheng, Jing

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