Synapsin III: Developmental expression, subcellular localization, and role in axon formation

Adriana Ferreira*, Hung Teh Kao, Jian Feng, Mark Rapoport, Paul Greengard

*Corresponding author for this work

Research output: Contribution to journalArticle

95 Scopus citations

Abstract

We have investigated the developmental expression and subcellular localization of synapsin III, the newest member of the synapsin family, in cultured mouse hippocampal neurons. Our results indicate that synapsin III is expressed early during development, with levels peaking 7 d after plating and declining thereafter. Synapsin III is highly concentrated in growth cones. Using specific antisense oligonucleotides, we have also examined the effect of depleting synapsin III on neurite elongation and synaptogenesis. When synapsin III was suppressed immediately after plating, hippocampal neurons extended minor processes but failed to differentiate one of them as the axon. The suppression of synapsin III after axonal elongation did not affect the time course of synapse formation. The results indicate that synapsin III has a developmental time course, a subcellular localization, and a developmental function very different from those of synapsin I and synapsin II.

Original languageEnglish (US)
Pages (from-to)3736-3744
Number of pages9
JournalJournal of Neuroscience
Volume20
Issue number10
StatePublished - May 15 2000

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Keywords

  • Antisense oligonucleotides
  • Axonal elongation
  • Growth cones
  • Neuronal polarity
  • Synapsin III
  • Synaptogenesis

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ferreira, A., Kao, H. T., Feng, J., Rapoport, M., & Greengard, P. (2000). Synapsin III: Developmental expression, subcellular localization, and role in axon formation. Journal of Neuroscience, 20(10), 3736-3744.