Synergistic simultaneous nitrification-endogenous denitrification and EBPR for advanced nitrogen and phosphorus removal in constructed wetlands

Jie Wang; Ling Xia; Jieyu Chen; Xiaoning Wang; Hu Wu; Dapeng Li; George F. Wells; Jun Yang; Jie Hou; Xugang He

doi:10.1016/j.cej.2020.127605

Synergistic simultaneous nitrification-endogenous denitrification and EBPR for advanced nitrogen and phosphorus removal in constructed wetlands

Jie Wang, Ling Xia, Jieyu Chen, Xiaoning Wang, Hu Wu, Dapeng Li, George F. Wells, Jun Yang, Jie Hou^*, Xugang He

^*Corresponding author for this work

Civil and Environmental Engineering

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

This study assessed the feasibility of achieving phosphorus removal through enhanced biological phosphorus removal (EBPR) in an intermittent aeration constructed wetland (CW). The results showed that within just 3 weeks, a polyphosphate-accumulating organisms (PAOs)-enriched biofilm was successfully established in the CW by integrating a pre-anaerobic stage, intermittent aeration and periodic PO₄^3--P release strategies. This strategy enabled a high PO₄^3--P removal efficiency of 92.68% using normal gravel as a substrate. Moreover, simultaneous nitrification-endogenous denitrification (SNED) was found to be the main nitrogen removal pathway in the EBPR-CW without specific control. SNED significantly reduces the demand for oxygen and organic carbon compared with classical nitrification-denitrification, and allowed the system to reach 96.21% total nitrogen (TN) removal even under carbon limiting conditions. With control experiment, EBPR combined with SNED in CWs improved the phosphorus and nitrogen removal by about 73% and 48%, respectively, compared with normal nitrification-denitrification and media storage-based nitrogen and phosphorus removal processes. Based on stoichiometric analysis, 71.25% of PO₄^3--P was removed by aerobic PAOs (APAOs), with 27.50% removed by DPAO_Ni (denitrifying PAOs using nitrite as electron acceptor) and 1.25% removed by DPAO_Na (denitrifying PAOs using nitrate as electron acceptor). DGAOs were the main organisms providing nitrite to DPAOs. Overall, our results demonstrate a novel approach to combine SNED with EBPR for advanced nitrogen and phosphorus removal in CWs.

Original language	English (US)
Article number	127605
Journal	Chemical Engineering Journal
Volume	420
DOIs	https://doi.org/10.1016/j.cej.2020.127605
State	Published - Sep 15 2021

Funding

This work was supported by Grants from National Natural Science Foundation of China (31972797); National Key Research and Development Program of China (2019YFD0900302); National Natural Science Foundation of China (51508219); the Open Subject of the State Key Laboratory of Freshwater Ecology and Biotechnology of the Institute of Hydrobiology of the Chinese Academy of Sciences (2017FB13); China Agriculture Research System (CARS-45).

Keywords

Constructed wetlands
EBPR
Nitrogen and phosphorus removal
Simultaneous nitrification-endogenous denitrification

ASJC Scopus subject areas

General Chemistry
Environmental Chemistry
General Chemical Engineering
Industrial and Manufacturing Engineering

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10.1016/j.cej.2020.127605

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@article{d2595539194a494f881e0353c27a09e7,

title = "Synergistic simultaneous nitrification-endogenous denitrification and EBPR for advanced nitrogen and phosphorus removal in constructed wetlands",

abstract = "This study assessed the feasibility of achieving phosphorus removal through enhanced biological phosphorus removal (EBPR) in an intermittent aeration constructed wetland (CW). The results showed that within just 3 weeks, a polyphosphate-accumulating organisms (PAOs)-enriched biofilm was successfully established in the CW by integrating a pre-anaerobic stage, intermittent aeration and periodic PO43--P release strategies. This strategy enabled a high PO43--P removal efficiency of 92.68% using normal gravel as a substrate. Moreover, simultaneous nitrification-endogenous denitrification (SNED) was found to be the main nitrogen removal pathway in the EBPR-CW without specific control. SNED significantly reduces the demand for oxygen and organic carbon compared with classical nitrification-denitrification, and allowed the system to reach 96.21% total nitrogen (TN) removal even under carbon limiting conditions. With control experiment, EBPR combined with SNED in CWs improved the phosphorus and nitrogen removal by about 73% and 48%, respectively, compared with normal nitrification-denitrification and media storage-based nitrogen and phosphorus removal processes. Based on stoichiometric analysis, 71.25% of PO43--P was removed by aerobic PAOs (APAOs), with 27.50% removed by DPAONi (denitrifying PAOs using nitrite as electron acceptor) and 1.25% removed by DPAONa (denitrifying PAOs using nitrate as electron acceptor). DGAOs were the main organisms providing nitrite to DPAOs. Overall, our results demonstrate a novel approach to combine SNED with EBPR for advanced nitrogen and phosphorus removal in CWs.",

keywords = "Constructed wetlands, EBPR, Nitrogen and phosphorus removal, Simultaneous nitrification-endogenous denitrification",

author = "Jie Wang and Ling Xia and Jieyu Chen and Xiaoning Wang and Hu Wu and Dapeng Li and Wells, {George F.} and Jun Yang and Jie Hou and Xugang He",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

year = "2021",

month = sep,

day = "15",

doi = "10.1016/j.cej.2020.127605",

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T1 - Synergistic simultaneous nitrification-endogenous denitrification and EBPR for advanced nitrogen and phosphorus removal in constructed wetlands

AU - Wang, Jie

AU - Xia, Ling

AU - Chen, Jieyu

AU - Wang, Xiaoning

AU - Wu, Hu

AU - Li, Dapeng

AU - Wells, George F.

AU - Yang, Jun

AU - Hou, Jie

AU - He, Xugang

PY - 2021/9/15

Y1 - 2021/9/15

N2 - This study assessed the feasibility of achieving phosphorus removal through enhanced biological phosphorus removal (EBPR) in an intermittent aeration constructed wetland (CW). The results showed that within just 3 weeks, a polyphosphate-accumulating organisms (PAOs)-enriched biofilm was successfully established in the CW by integrating a pre-anaerobic stage, intermittent aeration and periodic PO43--P release strategies. This strategy enabled a high PO43--P removal efficiency of 92.68% using normal gravel as a substrate. Moreover, simultaneous nitrification-endogenous denitrification (SNED) was found to be the main nitrogen removal pathway in the EBPR-CW without specific control. SNED significantly reduces the demand for oxygen and organic carbon compared with classical nitrification-denitrification, and allowed the system to reach 96.21% total nitrogen (TN) removal even under carbon limiting conditions. With control experiment, EBPR combined with SNED in CWs improved the phosphorus and nitrogen removal by about 73% and 48%, respectively, compared with normal nitrification-denitrification and media storage-based nitrogen and phosphorus removal processes. Based on stoichiometric analysis, 71.25% of PO43--P was removed by aerobic PAOs (APAOs), with 27.50% removed by DPAONi (denitrifying PAOs using nitrite as electron acceptor) and 1.25% removed by DPAONa (denitrifying PAOs using nitrate as electron acceptor). DGAOs were the main organisms providing nitrite to DPAOs. Overall, our results demonstrate a novel approach to combine SNED with EBPR for advanced nitrogen and phosphorus removal in CWs.

AB - This study assessed the feasibility of achieving phosphorus removal through enhanced biological phosphorus removal (EBPR) in an intermittent aeration constructed wetland (CW). The results showed that within just 3 weeks, a polyphosphate-accumulating organisms (PAOs)-enriched biofilm was successfully established in the CW by integrating a pre-anaerobic stage, intermittent aeration and periodic PO43--P release strategies. This strategy enabled a high PO43--P removal efficiency of 92.68% using normal gravel as a substrate. Moreover, simultaneous nitrification-endogenous denitrification (SNED) was found to be the main nitrogen removal pathway in the EBPR-CW without specific control. SNED significantly reduces the demand for oxygen and organic carbon compared with classical nitrification-denitrification, and allowed the system to reach 96.21% total nitrogen (TN) removal even under carbon limiting conditions. With control experiment, EBPR combined with SNED in CWs improved the phosphorus and nitrogen removal by about 73% and 48%, respectively, compared with normal nitrification-denitrification and media storage-based nitrogen and phosphorus removal processes. Based on stoichiometric analysis, 71.25% of PO43--P was removed by aerobic PAOs (APAOs), with 27.50% removed by DPAONi (denitrifying PAOs using nitrite as electron acceptor) and 1.25% removed by DPAONa (denitrifying PAOs using nitrate as electron acceptor). DGAOs were the main organisms providing nitrite to DPAOs. Overall, our results demonstrate a novel approach to combine SNED with EBPR for advanced nitrogen and phosphorus removal in CWs.

KW - Constructed wetlands

KW - EBPR

KW - Nitrogen and phosphorus removal

KW - Simultaneous nitrification-endogenous denitrification

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Synergistic simultaneous nitrification-endogenous denitrification and EBPR for advanced nitrogen and phosphorus removal in constructed wetlands

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