Synthesis and characterization of nano ceria for biological applications

Hsueh Shih Chen, Julie Mazzolini, Jon Ayres, Josh Rappoport, Jamie Lead, Jon Preece

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations


We have synthesized ceria nanoparticles by reacting cerium salts and sodium hydroxide without any additive in order to avoid possible cytotoxicity. The synthesized ceria nanoparticles were with cubic structure according to X-ray diffraction and have diameters between 6 and 10 nm analyzed by transmission electron microscopy. Aggregation behaviors of ceria nanoparticles in DI water and cell culture media have been studied by dynamic light scattering. In de-ionized water, ceria nanoparticles formed a stable colloidal solution with hydrodynamic size ~ 70 nm and zeta potential ~ 47 mV. Dispersion of the nanoparticles in serum-free media was found to result in larger aggregates, while the nanoparticles in serumcontaining media form a stable solution, which suggests that proteins in serum and ceria nanoparticles have some interactions. In cellular uptake test, entry of ceria nanoparticles into HeLa cells incubated in serum-containing media at 37 °C for 60 min was observed and without cytotoxicity.

Original languageEnglish (US)
Title of host publicationNano-Bio Sensing, Imaging, and Spectroscopy
StatePublished - Sep 17 2013
EventSPIE Nano-Bio Sensing, Imaging, and Spectroscopy - Jeju, Korea, Republic of
Duration: Feb 20 2013Feb 23 2013


OtherSPIE Nano-Bio Sensing, Imaging, and Spectroscopy
CountryKorea, Republic of


  • CeO
  • Ceria
  • Hydrothermal
  • Nanoparticles
  • Nanorods
  • Nanotoxicity
  • Nanowires
  • Toxicity

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Chen, H. S., Mazzolini, J., Ayres, J., Rappoport, J., Lead, J., & Preece, J. (2013). Synthesis and characterization of nano ceria for biological applications. In Nano-Bio Sensing, Imaging, and Spectroscopy (Vol. 8879). [887910]