Synthesis, characterization, and in vitro testing of a bacteria-targeted MR contrast agent

Lauren M. Matosziuk, Allison S. Harney, Keith W. MacRenaris, Thomas J. Meade*

*Corresponding author for this work

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A bacteria-targeted MR contrast agent, Zn-1, consisting of two Zn-dipicolylamine (Zn-dpa) groups conjugated to a Gd III chelate has been synthesized and characterized. In vitro studies with S. aureus and E. coli show that Zn-1 exhibits a significant improvement in bacteria labeling efficiency vs. control. Studies with a structural analogue, Zn-2, indicate that removal of one Zn-dpa moiety dramatically reduces the agent's affinity for bacteria. The ability of Zn-1 to significantly reduce the T 1 of labeled vs. unlabeled bacteria, resulting in enhanced MR image contrast, demonstrates its potential for visualizing bacterial infections in vivo. A bacteria-targeted MR contrast agent consisting of two Zn-dipicolylamine (Zn-dpa) groups conjugated to a Gd(III) chelate has been synthesized and characterized. This contrast agent significantly reduces the T 1 of labeled versus unlabeled bacteria, resulting in enhanced MR image contrast, demonstrating its potential for visualizing bacterial infections in vivo.

Original languageEnglish (US)
Pages (from-to)2099-2107
Number of pages9
JournalEuropean Journal of Inorganic Chemistry
Issue number12
DOIs
StatePublished - Apr 1 2012

Keywords

  • Bacteria labeling
  • Imaging agents
  • Magnetic resonance imaging
  • Medicinal chemistry
  • Zinc

ASJC Scopus subject areas

  • Inorganic Chemistry

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