@article{4a9272430e7443d88dd5b11965310b02,
title = "Synthesis of a human insulin gene V. Enzymatic assembly, cloning and characterization of the human proinsulin DNA",
abstract = "To form a 258-bp sequence coding for human proinsulin, 41 synthetic deoxyribo-oligonucleotide fragments of 11 to 15 nucleotides in length were assembled by enzymatic methods. The coding sequence is preceded by ATG and followed by TGA for translation start and stop signals, and terminated in an EcoRI and a BamHI recognition sequence. The complete synthetic sequence was ligated to a plasmid and cloned in Escherichia coli. The cloned DNA was shown to have the correct human proinsulin coding sequence.",
keywords = "Recombinant DNA, plasmid vectors, start and stop signals",
author = "R. Brousseau and R. Scarpulla and W. Sung and Hsiung, {H. M.} and Narang, {S. A.} and Ray Wu",
note = "Funding Information: We wish to thank Douglas H. Smith and J. Michniewicz for excellent technicaals sistance and Li-He Guo for valuable help with the sequence analysis of the cloned proinsulin gene. The work was supported by research grant AM21801 from the National Institute of Health, U.S. Public Health Service, from the National Research Council of Canada (NRCC NO 19599)a nd a fellowship grant DRG-323F (to R.C.S.) from the Damon Runyon-Walter Winchell Cancer Fund.",
year = "1982",
month = mar,
doi = "10.1016/0378-1119(82)90144-5",
language = "English (US)",
volume = "17",
pages = "279--289",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "3",
}