Synthesis of influenza virus polypeptides in cells resistant to alpha-amanitin: evidence for the involvement of cellular RNA polymerase II in virus replication

R. A. Lamb, P. W. Choppin

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Influenza virus polypeptides were not synthesized in wild-type CHO-S-infected cells in the presence of α-amanitin, but were synthesized in CHO-Ama1 cells, a mutant cell line whose DNA-dependent RNA polymerase II is specifically resistant to this drug, indicating that this cellular enzyme is involved in influenza virus replication. The results of experiments designed to detect viral polypeptides synthesized from primary transcripts suggest that the synthesis of a cellular RNA species by RNA polymerase II is required for primary transcription of the influenza virus genome.

Original languageEnglish (US)
Pages (from-to)816-819
Number of pages4
JournalJournal of virology
Volume23
Issue number3
DOIs
StatePublished - 1977

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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