Synthesis of the human insulin gene. Part IV. New synthetic deoxyribooligonucleotide adaptors and primer for DNA cloning and sequence analysis.

S. A. Narang*, R. Brousseau, H. M. Hsiung, W. Sung, R. Scarpulla, G. Ghangas, L. Lau, B. Hess, R. Wu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The chemical synthesis of four new deoxyribooligonucleotides to be used as adaptors in molecular cloning of DNA for expression studies is described. These are (i) start-adaptor, (ii) stop-adaptor, (iii) conversion adaptor to insert ribosomal binding site, and (iv) retrieving adaptor. We have also synthesized a 19-bases-long primer, 5'-TTGTAAAACGACGGCCAGT-3' to increase the speed of DNA sequence analysis and also demonstrated its application using single-stranded M13 DNa vector.

Original languageEnglish (US)
Pages (from-to)377-385
Number of pages9
JournalNucleic acids symposium series
Issue number7
StatePublished - Dec 1 1980

ASJC Scopus subject areas

  • Medicine(all)

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