Synthesis of the human insulin gene. Part IV. New synthetic deoxyribooligonucleotide adaptors and primer for DNA cloning and sequence analysis.

S. A. Narang; R. Brousseau; H. M. Hsiung; W. Sung; R. Scarpulla; G. Ghangas; L. Lau; B. Hess; R. Wu

Synthesis of the human insulin gene. Part IV. New synthetic deoxyribooligonucleotide adaptors and primer for DNA cloning and sequence analysis.

S. A. Narang^*, R. Brousseau, H. M. Hsiung, W. Sung, R. Scarpulla, G. Ghangas, L. Lau, B. Hess, R. Wu

^*Corresponding author for this work

Cell & Developmental Biology

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8 Scopus citations

Abstract

The chemical synthesis of four new deoxyribooligonucleotides to be used as adaptors in molecular cloning of DNA for expression studies is described. These are (i) start-adaptor, (ii) stop-adaptor, (iii) conversion adaptor to insert ribosomal binding site, and (iv) retrieving adaptor. We have also synthesized a 19-bases-long primer, 5'-TTGTAAAACGACGGCCAGT-3' to increase the speed of DNA sequence analysis and also demonstrated its application using single-stranded M13 DNa vector.

Original language	English (US)
Pages (from-to)	377-385
Number of pages	9
Journal	Nucleic acids symposium series
Issue number	7
State	Published - 1980

ASJC Scopus subject areas

General Medicine

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T1 - Synthesis of the human insulin gene. Part IV. New synthetic deoxyribooligonucleotide adaptors and primer for DNA cloning and sequence analysis.

AU - Narang, S. A.

AU - Brousseau, R.

AU - Hsiung, H. M.

AU - Sung, W.

AU - Scarpulla, R.

AU - Ghangas, G.

AU - Lau, L.

AU - Hess, B.

AU - Wu, R.

PY - 1980

Y1 - 1980

N2 - The chemical synthesis of four new deoxyribooligonucleotides to be used as adaptors in molecular cloning of DNA for expression studies is described. These are (i) start-adaptor, (ii) stop-adaptor, (iii) conversion adaptor to insert ribosomal binding site, and (iv) retrieving adaptor. We have also synthesized a 19-bases-long primer, 5'-TTGTAAAACGACGGCCAGT-3' to increase the speed of DNA sequence analysis and also demonstrated its application using single-stranded M13 DNa vector.

AB - The chemical synthesis of four new deoxyribooligonucleotides to be used as adaptors in molecular cloning of DNA for expression studies is described. These are (i) start-adaptor, (ii) stop-adaptor, (iii) conversion adaptor to insert ribosomal binding site, and (iv) retrieving adaptor. We have also synthesized a 19-bases-long primer, 5'-TTGTAAAACGACGGCCAGT-3' to increase the speed of DNA sequence analysis and also demonstrated its application using single-stranded M13 DNa vector.

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AN - SCOPUS:0019248020

SN - 0261-3166

SP - 377

EP - 385

JO - Nucleic acids symposium series

JF - Nucleic acids symposium series

IS - 7

ER -

Synthesis of the human insulin gene. Part IV. New synthetic deoxyribooligonucleotide adaptors and primer for DNA cloning and sequence analysis.

Abstract

ASJC Scopus subject areas

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