Synthetically programmable DNA binding domains in aggregates of DNA-functionalized gold nanoparticles

Sarah J. Hurst, Haley D. Hill, Robert J. Macfarlane, Jinsong Wu, Vinayak P. Dravid, Chad A. Mirkin

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

The system of DNA-Au NPs that can he used to create complex aggregate structures with two DNA binding domains unlike their conventional counterparts, which comprised a single binding domain and display a single, sharp melting transition upon dehybridization was designed. Many weak interactions become cumulatively strong in the context of these polyvalent oligonucleotide nanoparticle conjugates. The core/shell aggregate structures exhibit distinct melting transitions representative of a phase-separated structure and can be prepared in a variety of different ways from randomly dispersed particle buildinghlocks. The results revealed that the experiment can be used as a blueprint to synthesize even more complex DNA-linked nanoparticle aggregate structures with more than two binding domains, which further will also be useful to focus on using these materials to create controlled-release systems.

Original languageEnglish (US)
Pages (from-to)2156-2161
Number of pages6
JournalSmall
Volume5
Issue number19
DOIs
StatePublished - 2009

Keywords

  • DNA
  • Gold
  • Melting transitions
  • Nanoparticle conjugates

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

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