Systematic test of neurotoxin dose and volume on muscle function in a rat model

Jonah B. Hulst, Viviane B. Minamoto, Michael B. Lim, Shannon N. Bremner, Samuel R. Ward, Richard L. Lieber*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Introduction: Onabotulinum toxin serotype A (BT-A) is used for a variety of motor and sensory disorders related to abnormal muscle activity. Methods: We developed a high-resolution rodent model to allow precise determination of the effect of BT-A dose (measured in units) and injectate volume (measured in μl) on the efficacy of the injection and systemic side effects. Dorsiflexion is the best indicator of injected and contralateral muscle function. Results: One month after injection, dorsiflexion torque of BT-A-injected limbs was decreased significantly in all experimental groups compared with saline controls (P<0.05). Torque was also compared among the BT-A groups, which demonstrated a significant effect of dose (P<0.001), but no effect of volume (P>0.2) and no dose × volume interaction (P>0.3). Similar results were observed for other parameters measured. Conclusions: These data demonstrate that injection dose and not volume or concentration is the primary determinant of neurotoxin efficacy in a rodent model.

Original languageEnglish (US)
Pages (from-to)709-715
Number of pages7
JournalMuscle and Nerve
Volume49
Issue number5
DOIs
StatePublished - May 2014

Keywords

  • Cerebral palsy
  • Fibrosis
  • Myopathy
  • Onabotulinum toxin
  • Spasticity

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)

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