@article{386b6f7789eb45da9feafff5af8da354,
title = "Systemic infection facilitates transmission of Pseudomonas aeruginosa in mice",
abstract = "Health care-associated infections such as Pseudomonas aeruginosa bacteremia pose a major clinical risk for hospitalized patients. However, these systemic infections are presumed to be a “dead-end” for P. aeruginosa and to have no impact on transmission. Here, we use a mouse infection model to show that P. aeruginosa can spread from the bloodstream to the gallbladder, where it replicates to extremely high numbers. Bacteria in the gallbladder can then seed the intestines and feces, leading to transmission to uninfected cage-mate mice. Our work shows that the gallbladder is crucial for spread of P. aeruginosa from the bloodstream to the feces during bacteremia, a process that promotes transmission in this experimental system. Further research is needed to test to what extent these findings are relevant to infections in patients.",
author = "Bachta, {Kelly E.R.} and Allen, {Jonathan P.} and Cheung, {Bettina H.} and Chiu, {Cheng Hsun} and Hauser, {Alan R.}",
note = "Funding Information: We thank S. Abel and P. Abel for providing the scripts for pipeline analysis and discussion regarding STAMP, S. Lory, and J. Mekalanos for providing P. aeruginosa strains and cloning tools, L. Reynolds for technical assistance with electron microscopy, and S. Han and J. Zhang for mouse surgery. This work was supported by grants from the National Institutes of Health/National Institute of Allergy and Infectious Disease (R01 AI118257, R01 AI053674, U19 AI135964, K24 104831, and R21 AI129167, awarded to A.R.H.) an American Cancer Society Postdoctoral Fellowship (130602-PF-17-107-01-MPC, awarded to K.E.R.B.), and an American Heart Association Postdoctoral Fellowship (73POST25830019, awarded to J.P.A.). Additional support was provided by the North-western University Mouse Histology and Phenotyping Laboratory supported by an NCI Cancer Center Support Grant (CCSG NCI P30-CA060553). Imaging work was performed at the Northwestern Center for Advanced Microscopy (CAM) also generously supported by CCSG NCI P30 CA060553. Mouse surgery was performed by the North-western University Comprehensive Transplant Center (NUCTC) Microsurgery Core. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the paper. We would like to thank all members of the Hauser, Ozer, and Kociolek laboratories for their valuable comments during numerous discussions of this work. Publisher Copyright: {\textcopyright} 2020, The Author(s).",
year = "2020",
month = dec,
day = "1",
doi = "10.1038/s41467-020-14363-4",
language = "English (US)",
volume = "11",
journal = "Nature communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}