T cell antigen receptor engagement and specificity in the recognition of stress-inducible MHC class I-related chains by human epithelial γδ T cells

Jennifer Wu, Veronika Groh, Thomas Spies*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

211 Scopus citations

Abstract

Human γδ T cells with the TCR variable region Vδ1 occur mainly in epithelia and respond to stress-induced expression of the MHC class I-related chains A and B, which have no function in Ag presentation. MIC function as ligands for NKG2D-DAPI0, an activating receptor complex that triggers NK cells, costimulates CD8 αβ and Vγ9Vδ2 γδ T cells, and is required for stimulation of Vδ1 γδ T cells. It is unresolved, however, whether triggering of Vδ1 γδ TCRs is also mediated by MIC or by unidentified cell surface components. Soluble MICA tetramers were used as a binding reagent to demonstrate specific interactions with various Vδ1 γδ TCRs expressed on transfectants of a T cell line selected for lack of NKG2D. Tetramer binding was restricted to TCRs derived from responder T cell clones classified as reactive against a broad range of MIC-expressing target cells and was abrogated when TCRs were composed of mismatched γ- and δ-chains. These results and the inability of Vδ1 γδ T cells to respond to target cells expressing the ULBP/N2DL ligands of NKG2D, which are highly divergent from MIC, indicate that MIC delivers hoth the TCR-dependent signal I and the NKG2D-dependent costimulatory signal 2. This dual function may serve to prevent erroneous γδ T cell activation by cross-reactive cell surface determinants.

Original languageEnglish (US)
Pages (from-to)1236-1240
Number of pages5
JournalJournal of Immunology
Volume169
Issue number3
DOIs
StatePublished - Aug 1 2002

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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