A T cell hybridoma was developed that produced macrophage activation factor (MAF) but no gamma interferon (IFN(γ)). Hybridoma MAF was produced after stimulation with either concanavalin A (Con A) or phytohemagglutinin (PHA). Dose-response experiments showed that 1.5 μg/ml Con A provided maximum MAF production with equivalent levels of MAF produced at Con A concentrations up to 6 μg/ml. Time-course studied showed that maximum MAF activity was observed 48 hours after mitogen stimulation, and titration of MAF-containing supernatants showed that maximal MAF activity was observed at 1:4 dilutions with significant activity present at dilutions as great as 1:64. No IFN(γ) activity was detectable at any supernatant dilution.
|Original language||English (US)|
|Number of pages||5|
|Journal||RES Journal of the Reticuloendothelial Society|
|State||Published - Jan 1 1982|
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