T-cell hybridoma production of macrophage activation factor (MAF) I. Separation of MAF from interferon gamma

T. L. Ratliff, D. L. Thomasson, R. E. McCool, W. J. Catalona

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

A T cell hybridoma was developed that produced macrophage activation factor (MAF) but no gamma interferon (IFN(γ)). Hybridoma MAF was produced after stimulation with either concanavalin A (Con A) or phytohemagglutinin (PHA). Dose-response experiments showed that 1.5 μg/ml Con A provided maximum MAF production with equivalent levels of MAF produced at Con A concentrations up to 6 μg/ml. Time-course studied showed that maximum MAF activity was observed 48 hours after mitogen stimulation, and titration of MAF-containing supernatants showed that maximal MAF activity was observed at 1:4 dilutions with significant activity present at dilutions as great as 1:64. No IFN(γ) activity was detectable at any supernatant dilution.

Original languageEnglish (US)
Pages (from-to)393-397
Number of pages5
JournalRES Journal of the Reticuloendothelial Society
Volume31
Issue number5
StatePublished - Jan 1 1982

ASJC Scopus subject areas

  • Hematology

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