T cell proliferation and cytokine secretion to T cell epitopes of Asp f 2 in ABPA patients

Vipul B. Rathore*, Bryon Johnson, Jordan N. Fink, Kevin J. Kelly, Paul A. Greenberger, Viswanath P. Kurup

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The pathogenesis of allergic bronchopulmonary aspergillosis (ABPA) involves specific cytokines secreted by lymphocytes in response to Aspergillus fumigatus (Af) allergens. To gain information about the lymphoproliferative response and cytokine production against a major Af allergen, Asp f 2, we studied Asp-f-2-specific T cell clones (TCCs) from ABPA patients. TCCs were stimulated with rAsp f 2, its deletion mutants, and synthetic peptides to identify the T cell epitope(s) and to understand cytokine production. PBMCs from four of five ABPA patients showed proliferation in response to Asp f 2. Three TCCs from one patient showed higher IL-5 secretion compared to IL-4 and IFN-γ. Two TCCs from the second patient showed a mixed Th1/Th2 response, as evidenced by production of IL-4, IL-5, and IFN-γ. An epitope from the N-terminal region of Asp f 2 induced only IL-5 secretion. High IL-5 secretion might explain the marked eosinophilia observed in ABPA patients.

Original languageEnglish (US)
Pages (from-to)228-235
Number of pages8
JournalClinical Immunology
Volume100
Issue number2
DOIs
StatePublished - 2001

Keywords

  • ABPA
  • Allergy
  • Antigens/peptides/epitopes
  • Aspergillus
  • Cytokines
  • Th1/Th2

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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