T-cell receptor α-chain gene is split in a human T-cell leukemia cell line with a t(11;14)(p15;q11)

M. M. Le Beau, T. W. McKeithan, E. A. Shima, R. E. Goldman-Leikin, S. J. Chan, G. I. Bell, J. D. Rowley, M. O. Diaz

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Abstract

Chromosomal rearrangements in malignant T-cell disease frequently involve the chromosome bands containing the T-cell receptor genes. The RPMI 8402 cell line, which was established from the leukemia cells of a patient with T-cell acute lymphoblastic leukemia, is characterized by a translocation involving chromosome 14 (band q11) and chromosome 11 (band p15) [t(11;14)(p15;q11)]. By using in situ chromosomal hybridization and Southern blot analysis to examine RPMI 8402 cells, we determined that the break at 14q11 occurs within the variable region sequence of the T-cell receptor α-chain gene (TCRA); the break at 11p15 occurs between the HRAS1 gene and the genes for insulin and the insulin-like growth factor 2. These results suggest that the TCRA sequences activate a cellular gene located 11p15 in malignant T-cell disorders.

Original languageEnglish (US)
Pages (from-to)9744-9748
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number24
DOIs
StatePublished - Jan 1 1986

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