TY - JOUR
T1 - T-cell responses against chronic lymphocytic leukemia cells
T2 - Implications for immunotherapy
AU - Krackhardt, Angela M.
AU - Harig, Sabine
AU - Witzens, Mathias
AU - Broderick, Ryan
AU - Barrett, Patrick
AU - Gribben, John G.
PY - 2002/7/1
Y1 - 2002/7/1
N2 - Chronic lymphocytic leukemia (CLL) cells are ineffective antigen-presenting cells (APCs) although CD401-activated CLL cells can stimulate proliferation of autologous and allogeneic T cells. We examined the antigen-presenting capacity of CD40-activated CLL cells as well as dendritic cells pulsed with apoptotic bodies of CLL cells to generate autologous and allogeneic immune responses against CLL cells. Both APC types were capable of generating T-cell lines that proliferate specifically in response to unstimulated CLL cells. Whereas cytotoxic responses against stimulated and unstimulated CLL cells could be repeatedly generated by allogeneic healthy donors, autologous cytotoxic immune responses against CD40-activated and native CLL cells were rarely detected. However, T cells isolated from patients with CLL could recognize and lyse allogeneic stimulated and unstimulated CLL cells, demonstrating that cytotoxic T cells from these tumor-bearing patients are functionally intact.
AB - Chronic lymphocytic leukemia (CLL) cells are ineffective antigen-presenting cells (APCs) although CD401-activated CLL cells can stimulate proliferation of autologous and allogeneic T cells. We examined the antigen-presenting capacity of CD40-activated CLL cells as well as dendritic cells pulsed with apoptotic bodies of CLL cells to generate autologous and allogeneic immune responses against CLL cells. Both APC types were capable of generating T-cell lines that proliferate specifically in response to unstimulated CLL cells. Whereas cytotoxic responses against stimulated and unstimulated CLL cells could be repeatedly generated by allogeneic healthy donors, autologous cytotoxic immune responses against CD40-activated and native CLL cells were rarely detected. However, T cells isolated from patients with CLL could recognize and lyse allogeneic stimulated and unstimulated CLL cells, demonstrating that cytotoxic T cells from these tumor-bearing patients are functionally intact.
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U2 - 10.1182/blood.V100.1.167
DO - 10.1182/blood.V100.1.167
M3 - Article
C2 - 12070023
AN - SCOPUS:0036660173
SN - 0006-4971
VL - 100
SP - 167
EP - 173
JO - Blood
JF - Blood
IS - 1
ER -