T-cell responses against chronic lymphocytic leukemia cells: Implications for immunotherapy

Angela M. Krackhardt, Sabine Harig, Mathias Witzens, Ryan Broderick, Patrick Barrett, John G. Gribben*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

Chronic lymphocytic leukemia (CLL) cells are ineffective antigen-presenting cells (APCs) although CD401-activated CLL cells can stimulate proliferation of autologous and allogeneic T cells. We examined the antigen-presenting capacity of CD40-activated CLL cells as well as dendritic cells pulsed with apoptotic bodies of CLL cells to generate autologous and allogeneic immune responses against CLL cells. Both APC types were capable of generating T-cell lines that proliferate specifically in response to unstimulated CLL cells. Whereas cytotoxic responses against stimulated and unstimulated CLL cells could be repeatedly generated by allogeneic healthy donors, autologous cytotoxic immune responses against CD40-activated and native CLL cells were rarely detected. However, T cells isolated from patients with CLL could recognize and lyse allogeneic stimulated and unstimulated CLL cells, demonstrating that cytotoxic T cells from these tumor-bearing patients are functionally intact.

Original languageEnglish (US)
Pages (from-to)167-173
Number of pages7
JournalBlood
Volume100
Issue number1
DOIs
StatePublished - Jul 1 2002

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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