T Rosette assay In vitro correlate of cellular immunity

William J. Catalona; Claude Potvin; John L. Tarpley; Paul B. Chretien

doi:10.1016/0090-4295(74)90119-8

T Rosette assay In vitro correlate of cellular immunity

William J. Catalona^*, Claude Potvin, John L. Tarpley, Paul B. Chretien

^*Corresponding author for this work

Urology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The T rosette assay, based on the capacity of thymus-derived lymphocytes (mediators of tumor-inhibiting immunity) to bind sheep erythrocytes in vitro, was used to quantitate percentages of T rosette-forming lymphocytes in the peripheral blood of 38 patients with urologic cancer. Results obtained using the T rosette assay yielded statistically significant direct correlations with those obtained using two other assays which have been widely employed to monitor cellular immunity: elicitation of delayed hypersensitivity to DNCB (dinitrochlorobenzene) and the proliferative response of lymphocytes to PHA (phytohemagglutinin). The findings suggest the T rosette assay provides a meaningful in vitro correlate of cellular immunity.

Original language	English (US)
Pages (from-to)	100-104
Number of pages	5
Journal	Urology
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/0090-4295(74)90119-8
State	Published - Jul 1974

ASJC Scopus subject areas

Urology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0090-4295(74)90119-8

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title = "T Rosette assay In vitro correlate of cellular immunity",

abstract = "The T rosette assay, based on the capacity of thymus-derived lymphocytes (mediators of tumor-inhibiting immunity) to bind sheep erythrocytes in vitro, was used to quantitate percentages of T rosette-forming lymphocytes in the peripheral blood of 38 patients with urologic cancer. Results obtained using the T rosette assay yielded statistically significant direct correlations with those obtained using two other assays which have been widely employed to monitor cellular immunity: elicitation of delayed hypersensitivity to DNCB (dinitrochlorobenzene) and the proliferative response of lymphocytes to PHA (phytohemagglutinin). The findings suggest the T rosette assay provides a meaningful in vitro correlate of cellular immunity.",

author = "Catalona, {William J.} and Claude Potvin and Tarpley, {John L.} and Chretien, {Paul B.}",

note = "Funding Information: From the James Buchanan Brady Urological Institute, The Johns Hopkins Hospital, Baltimore, and Tumor Immunology Section, Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland",

year = "1974",

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doi = "10.1016/0090-4295(74)90119-8",

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T1 - T Rosette assay In vitro correlate of cellular immunity

AU - Catalona, William J.

AU - Potvin, Claude

AU - Tarpley, John L.

AU - Chretien, Paul B.

N1 - Funding Information: From the James Buchanan Brady Urological Institute, The Johns Hopkins Hospital, Baltimore, and Tumor Immunology Section, Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland

PY - 1974/7

Y1 - 1974/7

N2 - The T rosette assay, based on the capacity of thymus-derived lymphocytes (mediators of tumor-inhibiting immunity) to bind sheep erythrocytes in vitro, was used to quantitate percentages of T rosette-forming lymphocytes in the peripheral blood of 38 patients with urologic cancer. Results obtained using the T rosette assay yielded statistically significant direct correlations with those obtained using two other assays which have been widely employed to monitor cellular immunity: elicitation of delayed hypersensitivity to DNCB (dinitrochlorobenzene) and the proliferative response of lymphocytes to PHA (phytohemagglutinin). The findings suggest the T rosette assay provides a meaningful in vitro correlate of cellular immunity.

AB - The T rosette assay, based on the capacity of thymus-derived lymphocytes (mediators of tumor-inhibiting immunity) to bind sheep erythrocytes in vitro, was used to quantitate percentages of T rosette-forming lymphocytes in the peripheral blood of 38 patients with urologic cancer. Results obtained using the T rosette assay yielded statistically significant direct correlations with those obtained using two other assays which have been widely employed to monitor cellular immunity: elicitation of delayed hypersensitivity to DNCB (dinitrochlorobenzene) and the proliferative response of lymphocytes to PHA (phytohemagglutinin). The findings suggest the T rosette assay provides a meaningful in vitro correlate of cellular immunity.

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T Rosette assay In vitro correlate of cellular immunity

Abstract

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