T Rosette assay In vitro correlate of cellular immunity

William J. Catalona; Claude Potvin; John L. Tarpley; Paul B. Chretien

doi:10.1016/0090-4295(74)90119-8

T Rosette assay In vitro correlate of cellular immunity

William J. Catalona^*, Claude Potvin, John L. Tarpley, Paul B. Chretien

^*Corresponding author for this work

Urology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The T rosette assay, based on the capacity of thymus-derived lymphocytes (mediators of tumor-inhibiting immunity) to bind sheep erythrocytes in vitro, was used to quantitate percentages of T rosette-forming lymphocytes in the peripheral blood of 38 patients with urologic cancer. Results obtained using the T rosette assay yielded statistically significant direct correlations with those obtained using two other assays which have been widely employed to monitor cellular immunity: elicitation of delayed hypersensitivity to DNCB (dinitrochlorobenzene) and the proliferative response of lymphocytes to PHA (phytohemagglutinin). The findings suggest the T rosette assay provides a meaningful in vitro correlate of cellular immunity.

Original language	English (US)
Pages (from-to)	100-104
Number of pages	5
Journal	Urology
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/0090-4295(74)90119-8
State	Published - Jul 1974

Funding

From the James Buchanan Brady Urological Institute, The Johns Hopkins Hospital, Baltimore, and Tumor Immunology Section, Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland

ASJC Scopus subject areas

Urology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0090-4295(74)90119-8

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title = "T Rosette assay In vitro correlate of cellular immunity",

abstract = "The T rosette assay, based on the capacity of thymus-derived lymphocytes (mediators of tumor-inhibiting immunity) to bind sheep erythrocytes in vitro, was used to quantitate percentages of T rosette-forming lymphocytes in the peripheral blood of 38 patients with urologic cancer. Results obtained using the T rosette assay yielded statistically significant direct correlations with those obtained using two other assays which have been widely employed to monitor cellular immunity: elicitation of delayed hypersensitivity to DNCB (dinitrochlorobenzene) and the proliferative response of lymphocytes to PHA (phytohemagglutinin). The findings suggest the T rosette assay provides a meaningful in vitro correlate of cellular immunity.",

author = "Catalona, {William J.} and Claude Potvin and Tarpley, {John L.} and Chretien, {Paul B.}",

note = "Funding Information: From the James Buchanan Brady Urological Institute, The Johns Hopkins Hospital, Baltimore, and Tumor Immunology Section, Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland",

year = "1974",

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doi = "10.1016/0090-4295(74)90119-8",

language = "English (US)",

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T1 - T Rosette assay In vitro correlate of cellular immunity

AU - Catalona, William J.

AU - Potvin, Claude

AU - Tarpley, John L.

AU - Chretien, Paul B.

N1 - Funding Information: From the James Buchanan Brady Urological Institute, The Johns Hopkins Hospital, Baltimore, and Tumor Immunology Section, Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland

PY - 1974/7

Y1 - 1974/7

N2 - The T rosette assay, based on the capacity of thymus-derived lymphocytes (mediators of tumor-inhibiting immunity) to bind sheep erythrocytes in vitro, was used to quantitate percentages of T rosette-forming lymphocytes in the peripheral blood of 38 patients with urologic cancer. Results obtained using the T rosette assay yielded statistically significant direct correlations with those obtained using two other assays which have been widely employed to monitor cellular immunity: elicitation of delayed hypersensitivity to DNCB (dinitrochlorobenzene) and the proliferative response of lymphocytes to PHA (phytohemagglutinin). The findings suggest the T rosette assay provides a meaningful in vitro correlate of cellular immunity.

AB - The T rosette assay, based on the capacity of thymus-derived lymphocytes (mediators of tumor-inhibiting immunity) to bind sheep erythrocytes in vitro, was used to quantitate percentages of T rosette-forming lymphocytes in the peripheral blood of 38 patients with urologic cancer. Results obtained using the T rosette assay yielded statistically significant direct correlations with those obtained using two other assays which have been widely employed to monitor cellular immunity: elicitation of delayed hypersensitivity to DNCB (dinitrochlorobenzene) and the proliferative response of lymphocytes to PHA (phytohemagglutinin). The findings suggest the T rosette assay provides a meaningful in vitro correlate of cellular immunity.

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T Rosette assay In vitro correlate of cellular immunity

Abstract

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ASJC Scopus subject areas

UN SDGs

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