Tailoring DNA Structure to increase target hybridization kinetics on surfaces

Andrew E. Prigodich, One Sun Lee, Weston L. Daniel, Dwight S. Seferos, George C. Schatz, Chad A. Mirkin

Research output: Contribution to journalArticlepeer-review

65 Scopus citations


We report a method for increasing the rate of target hybridization on DNA-functionalized surfaces using a short internal complement DNA (sicDNA) strand. The sicDNA causes up to a 5-fold increase in association rate by inducing a conformational change that extends the DNA away from the surface, making it more available to bind target nucleic acids. The sicDNA-induced kinetic enhancement is a general phenomenon that occurred with all sequences and surfaces investigated. Additionally, the process is selective and can be used in multicomponent systems to controllably and orthogonally "turn on" specific sequences by the addition of the appropriate sicDNA. Finally, we show that sicDNA is compatible with systems used in gene regulation, intracellular detection, and microarrays, suggesting several potential therapeutic, diagnostic, and bioinformatic applications.

Original languageEnglish (US)
Pages (from-to)10638-10641
Number of pages4
JournalJournal of the American Chemical Society
Issue number31
StatePublished - Aug 11 2010

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry
  • Catalysis
  • Colloid and Surface Chemistry


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