TALEN-mediated editing of the mouse y chromosome

Haoyi Wang, Yueh Chiang Hu, Styliani Markoulaki, G. Grant Welstead, Albert W. Cheng, Chikdu S. Shivalila, Tatyana Pyntikova, Daniel B. Dadon, Daniel F. Voytas, Adam J. Bogdanove, David C. Page*, Rudolf Jaenisch

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

83 Scopus citations

Abstract

The functional study of Y chromosome genes has been hindered by a lack of mouse models with specific Y chromosome mutations. We used transcription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to produce mice with targeted gene disruptions and insertions in two Y-linked genes - Sry and Uty. TALEN-mediated gene editing is a useful tool for dissecting the biology of the Y chromosome.

Original languageEnglish (US)
Pages (from-to)530-532
Number of pages3
JournalNature biotechnology
Volume31
Issue number6
DOIs
StatePublished - Jun 2013

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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