Tapping a bacterial enzymatic pathway for the preparation and manipulation of synthetic nanomaterials

Ti Hsuan Ku, Swagat Sahu, Nicolas M. Kosa, Kevin M. Pham, Michael D. Burkart, Nathan C. Gianneschi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

We present a spherical micelle generated in a three-step sequence in which a farnesyl-pantetheine conjugate is phosphorylated, adenylated, and phosphorylated once more to generate a farnesyl-CoA amphiphile that self-assembles into spherical micelles. A sphere-to-fibril morphological switch is achieved by enzymatically transferring the farnesyl group of the farnesyl-CoA micelle onto a peptide via phosphopantetheinyl transferase to generate a peptide amphiphile. Each step in the sequence is followed with characterization by HPLC, MS, TEM, and DLS. This system offers an entry into cofactor-mediated peptide decoration by extending the principles of bioresponsive polymeric materials to sequential enzyme cascades.

Original languageEnglish (US)
Pages (from-to)17378-17381
Number of pages4
JournalJournal of the American Chemical Society
Volume136
Issue number50
DOIs
StatePublished - Dec 17 2014

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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