Teashirt is required for head-versus-tail regeneration polarity in planarians

Jared H. Owen, Daniel E. Wagner, Chun Chieh Chen, Christian P. Petersen, Peter W. Reddien*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Regeneration requires that the identities of new cells are properly specified to replace missing tissues. The Wnt signaling pathway serves a central role in specifying posterior cell fates during planarian regeneration. We identified a gene encoding a homolog of the Teashirt family of zinc-finger proteins in the planarian Schmidtea mediterranea to be a target of Wnt signaling in intact animals and at posterior-facing wounds. Inhibition of Smed-teashirt (teashirt) by RNA interference (RNAi) resulted in the regeneration of heads in place of tails, a phenotype previously observed with RNAi of the Wnt pathway genes β-catenin-1, wnt1, Dvl-1/2 or wntless. teashirt was required for β-catenin-1-dependent activation of posterior genes during regeneration. These findings identify teashirt as a transcriptional target of Wnt signaling required for Wnt-mediated specification of posterior blastemas.

Original languageEnglish (US)
Pages (from-to)1062-1072
Number of pages11
JournalDevelopment (Cambridge)
Volume142
Issue number6
DOIs
StatePublished - Mar 15 2015

Keywords

  • Neoblasts
  • Patterning
  • Planarians
  • Regeneration
  • Teashirt
  • Wnt

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Fingerprint Dive into the research topics of 'Teashirt is required for head-versus-tail regeneration polarity in planarians'. Together they form a unique fingerprint.

Cite this