Technical challenges in applying capillary electrophoresis-single strand conformation polymorphism for routine genetic analysis

Igor V Kourkine, Christa N. Hestekin, Annelise E. Barron*

*Corresponding author for this work

Research output: Contribution to journalReview article

50 Scopus citations

Abstract

Recent and future advances in population genetics will have a significant impact on health care practices and the economics of health care provision only if a spectrum of patient-tailored, effective methods of DNA screening for sequence alterations has been developed. Genetic screening by capillary electrophoresis-single strand conformation polymorphism (CE-SSCP), which is based upon the differences in electrophoretic mobilities of wild-type and mutant DNA species, offers an important complement to other presently available techniques such as Sanger sequencing and DNA hybridization arrays due to its simplicity, versatility, and low cost of analysis. A two-part review of CE-SSCP that discusses its advantages and limitations is presented. Emphasis is placed on technological aspects of CE-SSCP (including such rarely addressed issues as sample preparation protocols and the nature of the polymeric DNA separation matrix) as well as on the potential of CE-SSCP for routine genetic analysis. An attempt is made to organize and present the information in sufficient detail to allow the use of SSCP for routine genetic screening even by those inexperienced in CE. Some discussion of CE-based heteroduplex analysis (HA) is also presented.

Original languageEnglish (US)
Pages (from-to)1375-1385
Number of pages11
JournalELECTROPHORESIS
Volume23
Issue number10
DOIs
StatePublished - Jun 27 2002

Keywords

  • Capillary electrophoresis
  • Heteroduplex analysis
  • Mutation detection
  • Review
  • Single-strand conformation polymorphism

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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