TY - GEN
T1 - Technical considerations in quantitative blood oxygenation measurement using photoacoustic microscopy in vivo
AU - Maslov, Konstantin
AU - Sivaramakrishnan, Mathangi
AU - Zhang, Hao F.
AU - Stoica, George
AU - Wang, Lihong V.
PY - 2006
Y1 - 2006
N2 - Using peak amplitude spectral PA measurements in the range of 570 - 600 nm, we found it possible to quantify blood oxygenation levels. Visible light illumination minimizes the inversion error of the PA measurements. Owing to high blood absorption in this optical regime, there is also an improved signal-to-noise ratio and less influence from optical scattering. To arrive at correct, and vessel size independent, SO 2 measurements, the central frequency of the ultrasonic transducer must be high enough to satisfy the relation μ aΛ < 1, that is above 25 MHz for a chosen optical wavelength region, although lower frequency transducers may produce correct results after correction of the optical absorption spectra. However, additional efforts are needed to achieve accurate SO 2 for in vivo measurements.
AB - Using peak amplitude spectral PA measurements in the range of 570 - 600 nm, we found it possible to quantify blood oxygenation levels. Visible light illumination minimizes the inversion error of the PA measurements. Owing to high blood absorption in this optical regime, there is also an improved signal-to-noise ratio and less influence from optical scattering. To arrive at correct, and vessel size independent, SO 2 measurements, the central frequency of the ultrasonic transducer must be high enough to satisfy the relation μ aΛ < 1, that is above 25 MHz for a chosen optical wavelength region, although lower frequency transducers may produce correct results after correction of the optical absorption spectra. However, additional efforts are needed to achieve accurate SO 2 for in vivo measurements.
KW - Blood oxygenation
KW - Functional imaging
KW - Photoacoustic microscopy
KW - Spectroscopic imaging
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U2 - 10.1117/12.646265
DO - 10.1117/12.646265
M3 - Conference contribution
AN - SCOPUS:33646165063
SN - 0819461288
SN - 9780819461285
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Proceedings of SPIE - The International Society for Optical Engineering
T2 - 7th Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics - Photons Plus Ultrasound: Imaging and Sensing 2006
Y2 - 22 January 2006 through 26 January 2006
ER -