Technical considerations in quantitative blood oxygenation measurement using photoacoustic microscopy in vivo

Konstantin Maslov*, Mathangi Sivaramakrishnan, Hao F. Zhang, George Stoica, Lihong V. Wang

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

19 Scopus citations

Abstract

Using peak amplitude spectral PA measurements in the range of 570 - 600 nm, we found it possible to quantify blood oxygenation levels. Visible light illumination minimizes the inversion error of the PA measurements. Owing to high blood absorption in this optical regime, there is also an improved signal-to-noise ratio and less influence from optical scattering. To arrive at correct, and vessel size independent, SO 2 measurements, the central frequency of the ultrasonic transducer must be high enough to satisfy the relation μ aΛ < 1, that is above 25 MHz for a chosen optical wavelength region, although lower frequency transducers may produce correct results after correction of the optical absorption spectra. However, additional efforts are needed to achieve accurate SO 2 for in vivo measurements.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
DOIs
StatePublished - 2006
Event7th Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics - Photons Plus Ultrasound: Imaging and Sensing 2006 - San Jose, CA, United States
Duration: Jan 22 2006Jan 26 2006

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume6086
ISSN (Print)0277-786X

Other

Other7th Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics - Photons Plus Ultrasound: Imaging and Sensing 2006
CountryUnited States
CitySan Jose, CA
Period1/22/061/26/06

Keywords

  • Blood oxygenation
  • Functional imaging
  • Photoacoustic microscopy
  • Spectroscopic imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Maslov, K., Sivaramakrishnan, M., Zhang, H. F., Stoica, G., & Wang, L. V. (2006). Technical considerations in quantitative blood oxygenation measurement using photoacoustic microscopy in vivo. In Proceedings of SPIE - The International Society for Optical Engineering [60860R] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 6086). https://doi.org/10.1117/12.646265