Technical considerations in quantitative blood oxygenation measurement using photoacoustic microscopy in vivo

Konstantin Maslov; Mathangi Sivaramakrishnan; Hao F. Zhang; George Stoica; Lihong V. Wang

doi:10.1117/12.646265

Technical considerations in quantitative blood oxygenation measurement using photoacoustic microscopy in vivo

Konstantin Maslov^*, Mathangi Sivaramakrishnan, Hao F. Zhang, George Stoica, Lihong V. Wang

^*Corresponding author for this work

Biomedical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

20 Scopus citations

Abstract

Using peak amplitude spectral PA measurements in the range of 570 - 600 nm, we found it possible to quantify blood oxygenation levels. Visible light illumination minimizes the inversion error of the PA measurements. Owing to high blood absorption in this optical regime, there is also an improved signal-to-noise ratio and less influence from optical scattering. To arrive at correct, and vessel size independent, SO₂ measurements, the central frequency of the ultrasonic transducer must be high enough to satisfy the relation μ_aΛ < 1, that is above 25 MHz for a chosen optical wavelength region, although lower frequency transducers may produce correct results after correction of the optical absorption spectra. However, additional efforts are needed to achieve accurate SO₂ for in vivo measurements.

Original language	English (US)
Title of host publication	Photons Plus Ultrasound
Subtitle of host publication	Imaging and Sensing 2006 - The Seventh Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics
Publisher	SPIE
ISBN (Print)	0819461288, 9780819461285
DOIs	https://doi.org/10.1117/12.646265
State	Published - Feb 9 2006
Event	7th Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics - Photons Plus Ultrasound: Imaging and Sensing 2006 - San Jose, CA, United States Duration: Jan 22 2006 → Jan 26 2006

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	6086
ISSN (Print)	0277-786X

Other

Other	7th Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics - Photons Plus Ultrasound: Imaging and Sensing 2006
Country/Territory	United States
City	San Jose, CA
Period	1/22/06 → 1/26/06

Keywords

Blood oxygenation
Functional imaging
Photoacoustic microscopy
Spectroscopic imaging

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Access to Document

10.1117/12.646265

Cite this

Maslov, K., Sivaramakrishnan, M., Zhang, H. F., Stoica, G., & Wang, L. V. (2006). Technical considerations in quantitative blood oxygenation measurement using photoacoustic microscopy in vivo. In Photons Plus Ultrasound: Imaging and Sensing 2006 - The Seventh Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics Article 60860R (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 6086). SPIE. https://doi.org/10.1117/12.646265

Maslov, Konstantin ; Sivaramakrishnan, Mathangi ; Zhang, Hao F. et al. / Technical considerations in quantitative blood oxygenation measurement using photoacoustic microscopy in vivo. Photons Plus Ultrasound: Imaging and Sensing 2006 - The Seventh Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics. SPIE, 2006. (Proceedings of SPIE - The International Society for Optical Engineering).

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title = "Technical considerations in quantitative blood oxygenation measurement using photoacoustic microscopy in vivo",

abstract = "Using peak amplitude spectral PA measurements in the range of 570 - 600 nm, we found it possible to quantify blood oxygenation levels. Visible light illumination minimizes the inversion error of the PA measurements. Owing to high blood absorption in this optical regime, there is also an improved signal-to-noise ratio and less influence from optical scattering. To arrive at correct, and vessel size independent, SO2 measurements, the central frequency of the ultrasonic transducer must be high enough to satisfy the relation μaΛ < 1, that is above 25 MHz for a chosen optical wavelength region, although lower frequency transducers may produce correct results after correction of the optical absorption spectra. However, additional efforts are needed to achieve accurate SO2 for in vivo measurements.",

keywords = "Blood oxygenation, Functional imaging, Photoacoustic microscopy, Spectroscopic imaging",

author = "Konstantin Maslov and Mathangi Sivaramakrishnan and Zhang, {Hao F.} and George Stoica and Wang, {Lihong V.}",

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series = "Proceedings of SPIE - The International Society for Optical Engineering",

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booktitle = "Photons Plus Ultrasound",

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Maslov, K, Sivaramakrishnan, M, Zhang, HF, Stoica, G & Wang, LV 2006, Technical considerations in quantitative blood oxygenation measurement using photoacoustic microscopy in vivo. in Photons Plus Ultrasound: Imaging and Sensing 2006 - The Seventh Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics., 60860R, Proceedings of SPIE - The International Society for Optical Engineering, vol. 6086, SPIE, 7th Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics - Photons Plus Ultrasound: Imaging and Sensing 2006, San Jose, CA, United States, 1/22/06. https://doi.org/10.1117/12.646265

Technical considerations in quantitative blood oxygenation measurement using photoacoustic microscopy in vivo. / Maslov, Konstantin; Sivaramakrishnan, Mathangi; Zhang, Hao F. et al.
Photons Plus Ultrasound: Imaging and Sensing 2006 - The Seventh Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics. SPIE, 2006. 60860R (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 6086).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Technical considerations in quantitative blood oxygenation measurement using photoacoustic microscopy in vivo

AU - Maslov, Konstantin

AU - Sivaramakrishnan, Mathangi

AU - Zhang, Hao F.

AU - Stoica, George

AU - Wang, Lihong V.

PY - 2006/2/9

Y1 - 2006/2/9

N2 - Using peak amplitude spectral PA measurements in the range of 570 - 600 nm, we found it possible to quantify blood oxygenation levels. Visible light illumination minimizes the inversion error of the PA measurements. Owing to high blood absorption in this optical regime, there is also an improved signal-to-noise ratio and less influence from optical scattering. To arrive at correct, and vessel size independent, SO2 measurements, the central frequency of the ultrasonic transducer must be high enough to satisfy the relation μaΛ < 1, that is above 25 MHz for a chosen optical wavelength region, although lower frequency transducers may produce correct results after correction of the optical absorption spectra. However, additional efforts are needed to achieve accurate SO2 for in vivo measurements.

AB - Using peak amplitude spectral PA measurements in the range of 570 - 600 nm, we found it possible to quantify blood oxygenation levels. Visible light illumination minimizes the inversion error of the PA measurements. Owing to high blood absorption in this optical regime, there is also an improved signal-to-noise ratio and less influence from optical scattering. To arrive at correct, and vessel size independent, SO2 measurements, the central frequency of the ultrasonic transducer must be high enough to satisfy the relation μaΛ < 1, that is above 25 MHz for a chosen optical wavelength region, although lower frequency transducers may produce correct results after correction of the optical absorption spectra. However, additional efforts are needed to achieve accurate SO2 for in vivo measurements.

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KW - Functional imaging

KW - Photoacoustic microscopy

KW - Spectroscopic imaging

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T3 - Proceedings of SPIE - The International Society for Optical Engineering

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PB - SPIE

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Maslov K, Sivaramakrishnan M, Zhang HF, Stoica G, Wang LV. Technical considerations in quantitative blood oxygenation measurement using photoacoustic microscopy in vivo. In Photons Plus Ultrasound: Imaging and Sensing 2006 - The Seventh Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics. SPIE. 2006. 60860R. (Proceedings of SPIE - The International Society for Optical Engineering). doi: 10.1117/12.646265

Technical considerations in quantitative blood oxygenation measurement using photoacoustic microscopy in vivo

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