Template secondary structure promotes polymerase jumping during PCR amplification

V. K. Viswanathan, Kevin Krcmarik, Nicholas P. Cianciotto*

*Corresponding author for this work

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Pairs of primers flanking known miniTn10 transposon insertion sites were used to confirm the presence of the transposon in DNA isolated from Legionella pneumophila mutants. It was expected that the polymerase chain reaction products derived from the mutant template would be larger than those from the wild-type (WT) template due to the presence of the 1.8-kb transposon. Instead, it was observed that the mutant template yielded a product of almost the same size as that yielded by WT template. We present evidence to indicate that the aberrant product from the mutant template is a direct result of secondary structure of the template resulting from an inverted repeat sequence present in the miniTn10 transposon.

Original languageEnglish (US)
Pages (from-to)508-511
Number of pages4
JournalBioTechniques
Volume27
Issue number3
DOIs
StatePublished - 1999

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Template secondary structure promotes polymerase jumping during PCR amplification'. Together they form a unique fingerprint.

  • Cite this