Temporal and spatial transcriptomic and microRNA dynamics of CAM photosynthesis in pineapple

Ching M. Wai, Robert VanBuren, Jisen Zhang, Lixian Huang, Wenjing Miao, Patrick P. Edger, Won C. Yim, Henry D. Priest, Blake C. Meyers, Todd Mockler, J. Andrew C. Smith, John C. Cushman, Ray Ming*

*Corresponding author for this work

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

The altered carbon assimilation pathway of crassulacean acid metabolism (CAM) photosynthesis results in an up to 80% higher water-use efficiency than C3 photosynthesis in plants making it a potentially useful pathway for engineering crop plants with improved drought tolerance. Here we surveyed detailed temporal (diel time course) and spatial (across a leaf gradient) gene and microRNA (miRNA) expression patterns in the obligate CAM plant pineapple [Ananas comosus (L.) Merr.]. The high-resolution transcriptome atlas allowed us to distinguish between CAM-related and non-CAM gene copies. A differential gene co-expression network across green and white leaf diel datasets identified genes with circadian oscillation, CAM-related functions, and source-sink relations. Gene co-expression clusters containing CAM pathway genes are enriched with clock-associated cis-elements, suggesting circadian regulation of CAM. About 20% of pineapple microRNAs have diel expression patterns, with several that target key CAM-related genes. Expression and physiology data provide a model for CAM-specific carbohydrate flux and long-distance hexose transport. Together these resources provide a list of candidate genes for targeted engineering of CAM into C3 photosynthesis crop species.

Original languageEnglish (US)
Pages (from-to)19-30
Number of pages12
JournalPlant Journal
Volume92
Issue number1
DOIs
StatePublished - Oct 2017
Externally publishedYes

Keywords

  • Ananas comosus
  • CAM photosynthesis
  • circadian regulation
  • cis-element
  • stomata

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'Temporal and spatial transcriptomic and microRNA dynamics of CAM photosynthesis in pineapple'. Together they form a unique fingerprint.

  • Cite this

    Wai, C. M., VanBuren, R., Zhang, J., Huang, L., Miao, W., Edger, P. P., Yim, W. C., Priest, H. D., Meyers, B. C., Mockler, T., Smith, J. A. C., Cushman, J. C., & Ming, R. (2017). Temporal and spatial transcriptomic and microRNA dynamics of CAM photosynthesis in pineapple. Plant Journal, 92(1), 19-30. https://doi.org/10.1111/tpj.13630