Temporal profile of serum-induced S-phase entry and retinoblastoma protein phosphorylation in human skin fibroblasts

Farhad R. Danesh, Y. E. Minghao, Sasan Salmi, Michael Lapointe, Daniel Batlle*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Cultured skin fibroblasts from type 1 diabetic patients with nephropathy have a hyperplastic growth phenotype in comparison with diabetics without nephropathy and controls. We studied the G1 phase cyclins in skin fibroblasts from control subjects to define the temporal profile of serum-induced pRB phosphorylation, cyclin D1 protein expression, and cyclin D1/CDK4(6) kinase activity as well as S-phase entry by FACS analysis. Our preliminary studies indicate that cultured skin fibroblasts from type 1 diabetic patients with nephropathy have an enhanced pRB phosphorylation, cyclin D1 protein expression, and cyclin D1/CDK4(6) kinase activity. This finding may become useful to identify patients at risk for the development of nephropathy.

Original languageEnglish (US)
Pages (from-to)1282-1285
Number of pages4
JournalKidney international
Volume56
Issue number4
DOIs
StatePublished - 1999

Keywords

  • Cell growth
  • Glomerular mesangial cells
  • Hyperplastic growth phenotype
  • Nephropathy
  • Type 1 diabetes

ASJC Scopus subject areas

  • Nephrology

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