Temporal resolution of activity-dependent pH shifts in rat hippocampal slices

J. A. Gottfried, M. Chesler*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

1. The rise time of activity dependent extracellular pH shifts was measured in the CA1 stratum radiatum of rat hippocampal slices by recording pH sensitive fluorescence of a fluorescein-conjugated dextran. Optical data were compared with simultaneous pH microelectrode recordings. 2. The pH shifts generated by CO2 or by stimulation of the Schaffer collaterals were paralleled by shifts in fluorescence emissions at 535 nm when the probe was excited with 490-nm light (ΔF490). Emissions at 535 nm induced by 440-nm light were unchanged in these paradigms. 2. A train of three stimuli at 100 Hz was repeated at 30-s intervals and the stimulus-triggered ΔF490 was averaged. The mean rise time of the ΔF490 was 69 ± 24 (SE) ms (range 20- 200 ms, n = 6). The mean increase in emission was 0.75 ± 0.22% of baseline, associated with a pH microelectrode response of +0.06 ± 0.02 unit pH. 4. These data demonstrate that synaptically evoked alkaline transients develop within tens of milliseconds. The occurrence of the alkalinization in the same time frame as excitatory postsynaptic currents indicates that these pH shifts arise with sufficient speed to modulate synaptic transmission.

Original languageEnglish (US)
Pages (from-to)2804-2807
Number of pages4
JournalJournal of neurophysiology
Volume76
Issue number4
DOIs
StatePublished - Oct 1996

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

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