Testicular isoform of angiotensin I-converting enzyme (ACE, CD143) on the surface of human spermatozoa: Revelation and quantification using monoclonal antibodies

Marina A. Nikolaeva, Irina V. Balyasnikova, Marina A. Alexinskaya, Roman Metzger, Folker E. Franke, Ronald F. Albrecht, Vladimir I. Kulakov, Gennady T. Sukhikh, Sergei M. Danilov*

*Corresponding author for this work

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Problem: The elucidation of the role of angiotensin-converting enzyme (ACE, CD143) in the male fertility has been hampered by the absence of highly specific antibodies to the native testicular isoform (tACE). The quantification of tACE expression on human-ejaculated spermatozoa was performed using a novel panel of monoclonal antibodies (mAbs). Method of study: The expression of tACE on the surface of live and fixed human spermatozoa was analyzed by flow cytometry and immunocytochemistry using new mAbs to human tACE. Results: Monoclonal antibodies 1E10 and 4E3 similarly revealed tACE on the surface of live and fixed spermatozoa. The high percentage of tACEpositive spermatozoa (median 81%) was revealed in the swim-up fraction of sperm. Antibody-induced tACE shedding occurs preferentially from live sperm with defective function and/or morphology. Testicular ACE is located on the plasma membrane of the post-acrosomal region, the neck and midpiece of normal spermatozoa, but showed a variable distribution on the defective cells. Conclusions: The new mAbs recognizing the C-terminal domain of human ACE are useful tools for quantification of tACE expression on human live and fixed spermatozoa and further adequate analysis of the tACE role in reproduction.

Original languageEnglish (US)
Pages (from-to)54-68
Number of pages15
JournalAmerican Journal of Reproductive Immunology
Volume55
Issue number1
DOIs
StatePublished - Jan 1 2006

Keywords

  • Flow cytometry
  • Monoclonal antibodies
  • Spermatozoa
  • Testicular ACE

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynecology

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