Testing the critical size in calvarial bone defects: Revisiting the concept of a critical-size defect

Gregory M. Cooper, Mark P. Mooney, Arun K. Gosain, Phil G. Campbell, Joseph E. Losee, Johnny Huard

Research output: Contribution to journalArticlepeer-review

180 Scopus citations


BACKGROUND: There is a clinical need for bone replacement strategies because of the shortfalls endemic to autologous bone grafting, especially in the pediatric patient population. For the past 25 years, the animal model that has been used to test bone replacement strategies has been the calvarial critical-size defect, based on the initial size of the bone defect. This study was undertaken to test the concept of the critical size in several different models. A review of the theoretical and scientific bases for the critical-size defect was also undertaken. METHODS: Two different rodent species (including 28 adult mice and six adult rats) were used to assess bone healing by means of two-dimensional radiographic analysis after creating small bone defects using different surgical techniques. RESULTS: Defects in mice that were smaller than critical-size defects (1.8-mm diameter) were shown to heal a maximum of 50 percent 1 year postoperatively. Small defects (2.3-mm diameter) in the rat skull showed approximately 35 percent healing after 6 weeks. Neither the choice of rodent species nor the maintenance of the dura mater significantly affected calvarial bone healing. CONCLUSIONS: These results suggest that calvarial bone healing is not well described and much more data need to be collected. Also, after a review of the existing literature and a critique of the clinical applicability of the model, it is suggested that the use of the term "critical-size defect" be discontinued.

Original languageEnglish (US)
Pages (from-to)1685-1692
Number of pages8
JournalPlastic and reconstructive surgery
Issue number6
StatePublished - Jun 2010

ASJC Scopus subject areas

  • Surgery


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