Texture image analysis in differentiating malignant from benign adrenal cortical tumors in children and adults

N. K. Shirata, Simone Trieger Sredni, A. Castelo, A. Santinelli, B. Mendonça, R. Montironi, A. Longatto Filho, Maria Claudia Nogueira Zerbini

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Objective: To investigate the possible role of chromatin texture parameters, nuclear morphology, DNA ploidy and clinical functional status in discriminating benign from malignant adrenocortical tumors (ACT). Patients and Methods: Forty-eight cases of clinically benign (n=40) and clinically malignant (n=8) ACT with a minimum of 5-years' follow-up were evaluated for chromatin texture parameters (run length, standard deviation, configurable run length, valley, slope, peak and other 21 Markovian features that describe the distribution of the chromatin in the nucleus), nuclear morphology (nuclear area, nuclear perimeter, nuclear maximum and minumum diameter, nuclear shape), and DNA ploidy. Nuclear parameters were evaluated in Feulgen-stained 5 μm paraffin-sections analyzed using a CAS 200 image analyzer. Results: Since ACTs present different biological features in children and adults, patients were divided into two groups: children (≤ 15 years) and adults (>15 years). In the group of children DNA ploidy presented a marginal significance (p=0.05) in discriminating ACTs. None of the parameters discriminated between malignant and benign ACT in the adult group. Conclusion: ACTs are uncommon and definitive predictive criteria for malignancy remain uncertain, particularly in children. Our data point to DNA content evaluated by image analysis as a new candidate tool for this challenging task. Texture image analysis did not help to differentiate malignant from benign adrenal cortical tumors in children and adults.

Original languageEnglish (US)
Pages (from-to)3365-3368
Number of pages4
JournalAnticancer Research
Issue number8
StatePublished - Aug 1 2009


  • Adrenal
  • Adrenocortical tumors
  • Adults
  • Children
  • Chromatin texture
  • DNA ploidy
  • Image analysis
  • Nuclear morphology

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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