TGFβ1 modulates the phenotype of Schwann cells at the transcriptional level

Rajeshwar Awatramani; Susan Shumas; John Kamholz; Steven S. Scherer

doi:10.1006/mcne.2001.1094

TGFβ1 modulates the phenotype of Schwann cells at the transcriptional level

Rajeshwar Awatramani, Susan Shumas, John Kamholz, Steven S. Scherer^*

^*Corresponding author for this work

Neurology

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We have examined the effects of transforming growth factor β1 (TGFβ1) on gene expression in cultured rat Schwann cells (SCs). TGFβ1 decreased the steady-state mRNA levels of several genes that are expressed by myelinating SCs but had varied effects on the mRNA levels of NCAM, L1, GAP-43, and p75-genes that are expressed by denervated and nonmyelinating SCs. TGFβ1 antagonized the effects of forskolin on the mRNA levels of the transcription factors Oct-6/tst-1/SCIP and Krox20. Transcriptional run-off analysis demonstrated that the effects of TGFβ1 on gene expression occur at least in part at the level of transcription. Thus, TGFβ1 suppresses the expression of genes that characterize the different phenotypes of SCs, and these changes occur at least in part at a transcriptional level.

Original language	English (US)
Pages (from-to)	307-319
Number of pages	13
Journal	Molecular and Cellular Neuroscience
Volume	19
Issue number	3
DOIs	https://doi.org/10.1006/mcne.2001.1094
State	Published - 2002

Funding

This work was supported by the NIH (NS08075, NS37100, and NS34528 to S.S.S.) as well as the Muscular Dystrophy Association and the National Multiple Sclerosis Society (to J.K). We thank Dr. Mark Marchionni for the neuregulin-1 and numerous colleagues for their generous gifts of cDNAs and antibodies.

ASJC Scopus subject areas

Cellular and Molecular Neuroscience
Molecular Biology
Cell Biology

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10.1006/mcne.2001.1094

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title = "TGFβ1 modulates the phenotype of Schwann cells at the transcriptional level",

abstract = "We have examined the effects of transforming growth factor β1 (TGFβ1) on gene expression in cultured rat Schwann cells (SCs). TGFβ1 decreased the steady-state mRNA levels of several genes that are expressed by myelinating SCs but had varied effects on the mRNA levels of NCAM, L1, GAP-43, and p75-genes that are expressed by denervated and nonmyelinating SCs. TGFβ1 antagonized the effects of forskolin on the mRNA levels of the transcription factors Oct-6/tst-1/SCIP and Krox20. Transcriptional run-off analysis demonstrated that the effects of TGFβ1 on gene expression occur at least in part at the level of transcription. Thus, TGFβ1 suppresses the expression of genes that characterize the different phenotypes of SCs, and these changes occur at least in part at a transcriptional level.",

author = "Rajeshwar Awatramani and Susan Shumas and John Kamholz and Scherer, {Steven S.}",

note = "Funding Information: This work was supported by the NIH (NS08075, NS37100, and NS34528 to S.S.S.) as well as the Muscular Dystrophy Association and the National Multiple Sclerosis Society (to J.K). We thank Dr. Mark Marchionni for the neuregulin-1 and numerous colleagues for their generous gifts of cDNAs and antibodies.",

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AU - Shumas, Susan

AU - Kamholz, John

AU - Scherer, Steven S.

N1 - Funding Information: This work was supported by the NIH (NS08075, NS37100, and NS34528 to S.S.S.) as well as the Muscular Dystrophy Association and the National Multiple Sclerosis Society (to J.K). We thank Dr. Mark Marchionni for the neuregulin-1 and numerous colleagues for their generous gifts of cDNAs and antibodies.

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N2 - We have examined the effects of transforming growth factor β1 (TGFβ1) on gene expression in cultured rat Schwann cells (SCs). TGFβ1 decreased the steady-state mRNA levels of several genes that are expressed by myelinating SCs but had varied effects on the mRNA levels of NCAM, L1, GAP-43, and p75-genes that are expressed by denervated and nonmyelinating SCs. TGFβ1 antagonized the effects of forskolin on the mRNA levels of the transcription factors Oct-6/tst-1/SCIP and Krox20. Transcriptional run-off analysis demonstrated that the effects of TGFβ1 on gene expression occur at least in part at the level of transcription. Thus, TGFβ1 suppresses the expression of genes that characterize the different phenotypes of SCs, and these changes occur at least in part at a transcriptional level.

AB - We have examined the effects of transforming growth factor β1 (TGFβ1) on gene expression in cultured rat Schwann cells (SCs). TGFβ1 decreased the steady-state mRNA levels of several genes that are expressed by myelinating SCs but had varied effects on the mRNA levels of NCAM, L1, GAP-43, and p75-genes that are expressed by denervated and nonmyelinating SCs. TGFβ1 antagonized the effects of forskolin on the mRNA levels of the transcription factors Oct-6/tst-1/SCIP and Krox20. Transcriptional run-off analysis demonstrated that the effects of TGFβ1 on gene expression occur at least in part at the level of transcription. Thus, TGFβ1 suppresses the expression of genes that characterize the different phenotypes of SCs, and these changes occur at least in part at a transcriptional level.

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TGFβ1 modulates the phenotype of Schwann cells at the transcriptional level

Abstract

Funding

ASJC Scopus subject areas

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