TGFβ1 modulates the phenotype of Schwann cells at the transcriptional level

Rajeshwar Awatramani, Susan Shumas, John Kamholz, Steven S. Scherer*

*Corresponding author for this work

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

We have examined the effects of transforming growth factor β1 (TGFβ1) on gene expression in cultured rat Schwann cells (SCs). TGFβ1 decreased the steady-state mRNA levels of several genes that are expressed by myelinating SCs but had varied effects on the mRNA levels of NCAM, L1, GAP-43, and p75-genes that are expressed by denervated and nonmyelinating SCs. TGFβ1 antagonized the effects of forskolin on the mRNA levels of the transcription factors Oct-6/tst-1/SCIP and Krox20. Transcriptional run-off analysis demonstrated that the effects of TGFβ1 on gene expression occur at least in part at the level of transcription. Thus, TGFβ1 suppresses the expression of genes that characterize the different phenotypes of SCs, and these changes occur at least in part at a transcriptional level.

Original languageEnglish (US)
Pages (from-to)307-319
Number of pages13
JournalMolecular and Cellular Neuroscience
Volume19
Issue number3
DOIs
StatePublished - Jan 1 2002

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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