TGF-β mRNA expression in the renal pelvis after experimental and clinical ureteropelvic junction obstruction

G. M. Seremetis*, M. Maizels

*Corresponding author for this work

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Purpose: The renal parenchymal expression of transforming growth factor- beta (TGF-β) is increased in experimental obstructive uropathy. To better understand the pathophysiology of hydronephrosis, we pursued this observation by determining if the expression of TGF-β is also increased in the obstructed renal pelvis. Materials and Methods: The expression of TGF-β mRNA was evaluated in experimental and clinical ureteropelvic junction obstruction by reverse transcriptase polymerase chain reaction (RT-PCR). Southern blot analysis with labeled human cDNA probes for TGF-β was then done to verify the results. Results: Experimentally, pelves subjected to ureteral constriction showed significantly higher TGF-β mRNA expression than normals. After reversal of obstruction, the expression of TGF-β remained significantly higher than normals but was not different from pelves with ongoing obstruction. Clinically, there was a significantly higher level of TGF-β mRNA expression in obstructed pelves than in nonobstructed ureters. High levels of TGF-β mRNA expression correlated significantly with good clinical outcome, good renal function, muscle hypertrophy and acute onset of obstruction. Conclusions: There is increased TGF-β mRNA expression in the renal pelvis following clinical and experimental ureteropelvic junction obstruction. These data implicate TGF-β in the adaptive molecular responses that increase muscle and collagen elaboration seen in hydronephrosis.

Original languageEnglish (US)
Pages (from-to)261-266
Number of pages6
JournalJournal of Urology
Volume156
Issue number1
DOIs
StatePublished - Jan 1 1996

Keywords

  • growth substances
  • hydronephrosis
  • polymerase chain reaction

ASJC Scopus subject areas

  • Urology

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