The 14q22.2 colorectal cancer variant rs4444235 shows cis-acting regulation of BMP4

S. J. Lubbe, A. M. Pittman, B. Olver, A. Lloyd, J. Vijayakrishnan, S. Naranjo, S. Dobbins, P. Broderick, J. L. Gómez-Skarmeta, R. S. Houlston*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Common genetic variation at human 14q22.2 tagged by rs4444235 is significantly associated with colorectal cancer (CRC) risk. Re-sequencing was used to comprehensively annotate the 17kb region of strong linkage disequilibrium encompassing rs4444235. Through bioinformatic analyses using H3K4Me1, H3K4Me3, and DNase-I hypersensitivity chromatin signatures and evolutionary conservation we identified seven candidate disease-causing single-nucleotide polymorphisms mapping to six regions within the 17-kb region predicted to have regulatory potential. Reporter gene studies of these regions demonstrated that the element to which rs4444235 maps acts as an allele-specific transcriptional enhancer. Allele-specific expression studies in CRC cell lines heterozygous for rs4444235 showed significantly increased expression of bone morphogenetic protein-4 (BMP4) associated with the risk allele (P < 0.001). These data provide evidence for a functional basis for the non-coding risk variant rs4444235 at 14q22.2 and emphasizes the importance of genetic variation in the BMP pathway genes as determinants of CRC risk.

Original languageEnglish (US)
Pages (from-to)3777-3784
Number of pages8
JournalOncogene
Volume31
Issue number33
DOIs
StatePublished - Aug 16 2012

Keywords

  • bone morphogenetic protein-4
  • cis-regulatory
  • colorectal cancer

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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