TY - JOUR
T1 - The aged retinal pigment epithelium/choroid
T2 - A potential substratum for the pathogenesis of age-related macular degeneration
AU - Chen, Huiyi
AU - Liu, Bin
AU - Lukas, Thomas J.
AU - Neufeld, Arthur H.
PY - 2008/6/4
Y1 - 2008/6/4
N2 - Background: Although the statement that age is the greatest risk factor for Age-related macular degeneration (AMD) is widely accepted, the cellular and molecular explanation for that clinical statement are not generally known. A major focus of AMD research is the retinal pigment epithelium (RPE/choroid. The purpose of this study was to characterize the changes in the RPE/choroid with age that may provide a background for the development of AMD. Methodology/Principal Findings: We compared the transcriptional profiles, key protein levels and histology of the RPE/choroid from young and old mice. Using three statistical methods. microarray data demonstrated marked changes in the old mouse. There were 315 genes differentially expressed with age most of these genes were related to immune responses and inflammatory activity. Canonical pathways having significant numbers of upregulated genes in aged RPE/choroid included leukocyte extravasation, complement cascades, natural killer cell signaling and IL-10 signaling. By contrast, the adjacent neural retina showed completely different age-related changes. The levels of proteins that participate in leukocyte extravasation and complement pathways were consistently increased in the normal, aged RPE/choroid. Furthermore, there was increased gene expression and protein levels of leukocyte attracting signal, chemokine ligand 2 (Ccl2) in aged RPE/choroid. In old animals, there was marked extravasation and accumulation of leukocytes from the choroidal circulation onto Bruch's membrane and into the RPE. Conclusions/Significance: These phenotypic changes indicate that the RPE/ choroid in the normal, old mouse has become an immunologically active tissue. There are signals from the normal, aged RPE/choroid which recruit leukocytes from the circulation and activate the complement cascade. These age-related changes that occur in the RPE/choroid with age to the extent that they occur in the human retina, may provide the background for an error in regulation of immunological activity to cause AMD to appear in an elderly individual.
AB - Background: Although the statement that age is the greatest risk factor for Age-related macular degeneration (AMD) is widely accepted, the cellular and molecular explanation for that clinical statement are not generally known. A major focus of AMD research is the retinal pigment epithelium (RPE/choroid. The purpose of this study was to characterize the changes in the RPE/choroid with age that may provide a background for the development of AMD. Methodology/Principal Findings: We compared the transcriptional profiles, key protein levels and histology of the RPE/choroid from young and old mice. Using three statistical methods. microarray data demonstrated marked changes in the old mouse. There were 315 genes differentially expressed with age most of these genes were related to immune responses and inflammatory activity. Canonical pathways having significant numbers of upregulated genes in aged RPE/choroid included leukocyte extravasation, complement cascades, natural killer cell signaling and IL-10 signaling. By contrast, the adjacent neural retina showed completely different age-related changes. The levels of proteins that participate in leukocyte extravasation and complement pathways were consistently increased in the normal, aged RPE/choroid. Furthermore, there was increased gene expression and protein levels of leukocyte attracting signal, chemokine ligand 2 (Ccl2) in aged RPE/choroid. In old animals, there was marked extravasation and accumulation of leukocytes from the choroidal circulation onto Bruch's membrane and into the RPE. Conclusions/Significance: These phenotypic changes indicate that the RPE/ choroid in the normal, old mouse has become an immunologically active tissue. There are signals from the normal, aged RPE/choroid which recruit leukocytes from the circulation and activate the complement cascade. These age-related changes that occur in the RPE/choroid with age to the extent that they occur in the human retina, may provide the background for an error in regulation of immunological activity to cause AMD to appear in an elderly individual.
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U2 - 10.1371/journal.pone.0002339
DO - 10.1371/journal.pone.0002339
M3 - Article
C2 - 18523633
AN - SCOPUS:48649088785
VL - 3
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 6
M1 - e2339
ER -