The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis

Zijue Zhu, Wangjie Xu, Jingbo Dai, Xiaohui Chen, Xianglong Zhao, Peng Fang, Fang Yang, Maoping Tang, Zhaoxia Wang, Lianyun Wang, Duan Ma, Zhongdong Qaio*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Smoking is associated with a declining quality of semen. The aim of this study was to screen and investigate the differential expression of proteins extracted from the epididymis of mice exposed daily with cigarette smoke. Using MALDI-TOF-MS analysis, we found that the protein profile of the mouse epididymis was altered by cigarette smoking and identified 27 proteins from the most abundant and differentially expressed spots in the 2-DE gels of epididymal samples. These proteins were classified into groups according to their functions such as energy metabolism, reproduction and structural molecule activity. Through pathway analysis, these proteins were associated with the glutathione metabolism and protein processing in the endoplasmic reticulum. These results showed that the epididymis may experience oxidative stress following cigarette smoke exposure, which was confirmed using immunohistochemistry. We determine that cigarette smoking can induce oxidative stress in the mouse epididymis, which may cause protein profile altering, thereby impairing epididymis function, and leading to a decline in semen quality.

Original languageEnglish (US)
Pages (from-to)571-582
Number of pages12
JournalInternational Journal of Biochemistry and Cell Biology
Issue number3
StatePublished - Mar 2013


  • 2-D Gel electrophoresis
  • Cigarette smoking
  • Epididymis
  • Male fertility
  • Mass spectrometry

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology


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