The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis

Zijue Zhu; Wangjie Xu; Jingbo Dai; Xiaohui Chen; Xianglong Zhao; Peng Fang; Fang Yang; Maoping Tang; Zhaoxia Wang; Lianyun Wang; Duan Ma; Zhongdong Qaio

doi:10.1016/j.biocel.2012.12.007

The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis

Zijue Zhu, Wangjie Xu, Jingbo Dai, Xiaohui Chen, Xianglong Zhao, Peng Fang, Fang Yang, Maoping Tang, Zhaoxia Wang, Lianyun Wang, Duan Ma, Zhongdong Qaio^*

^*Corresponding author for this work

Pediatrics

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Smoking is associated with a declining quality of semen. The aim of this study was to screen and investigate the differential expression of proteins extracted from the epididymis of mice exposed daily with cigarette smoke. Using MALDI-TOF-MS analysis, we found that the protein profile of the mouse epididymis was altered by cigarette smoking and identified 27 proteins from the most abundant and differentially expressed spots in the 2-DE gels of epididymal samples. These proteins were classified into groups according to their functions such as energy metabolism, reproduction and structural molecule activity. Through pathway analysis, these proteins were associated with the glutathione metabolism and protein processing in the endoplasmic reticulum. These results showed that the epididymis may experience oxidative stress following cigarette smoke exposure, which was confirmed using immunohistochemistry. We determine that cigarette smoking can induce oxidative stress in the mouse epididymis, which may cause protein profile altering, thereby impairing epididymis function, and leading to a decline in semen quality.

Original language	English (US)
Pages (from-to)	571-582
Number of pages	12
Journal	International Journal of Biochemistry and Cell Biology
Volume	45
Issue number	3
DOIs	https://doi.org/10.1016/j.biocel.2012.12.007
State	Published - Mar 2013

Funding

This work was supported by the National Natural Science Foundation of China (No. 30901490 ), Medical Engineering in a joint project with Shanghai Jiao Tong University ( YG2010 MS27 ) and the National Key Basic Research Program ( 2009CB941704 ).

Keywords

2-D Gel electrophoresis
Cigarette smoking
Epididymis
Male fertility
Mass spectrometry

ASJC Scopus subject areas

Biochemistry
Cell Biology

Access to Document

10.1016/j.biocel.2012.12.007

Cite this

@article{64f78608fbe941a3a092ac893d702bf3,

title = "The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis",

abstract = "Smoking is associated with a declining quality of semen. The aim of this study was to screen and investigate the differential expression of proteins extracted from the epididymis of mice exposed daily with cigarette smoke. Using MALDI-TOF-MS analysis, we found that the protein profile of the mouse epididymis was altered by cigarette smoking and identified 27 proteins from the most abundant and differentially expressed spots in the 2-DE gels of epididymal samples. These proteins were classified into groups according to their functions such as energy metabolism, reproduction and structural molecule activity. Through pathway analysis, these proteins were associated with the glutathione metabolism and protein processing in the endoplasmic reticulum. These results showed that the epididymis may experience oxidative stress following cigarette smoke exposure, which was confirmed using immunohistochemistry. We determine that cigarette smoking can induce oxidative stress in the mouse epididymis, which may cause protein profile altering, thereby impairing epididymis function, and leading to a decline in semen quality.",

keywords = "2-D Gel electrophoresis, Cigarette smoking, Epididymis, Male fertility, Mass spectrometry",

author = "Zijue Zhu and Wangjie Xu and Jingbo Dai and Xiaohui Chen and Xianglong Zhao and Peng Fang and Fang Yang and Maoping Tang and Zhaoxia Wang and Lianyun Wang and Duan Ma and Zhongdong Qaio",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China (No. 30901490 ), Medical Engineering in a joint project with Shanghai Jiao Tong University ( YG2010 MS27 ) and the National Key Basic Research Program ( 2009CB941704 ).",

year = "2013",

month = mar,

doi = "10.1016/j.biocel.2012.12.007",

language = "English (US)",

volume = "45",

pages = "571--582",

journal = "International Journal of Biochemistry and Cell Biology",

issn = "1357-2725",

publisher = "Elsevier Ltd",

number = "3",

}

TY - JOUR

T1 - The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis

AU - Zhu, Zijue

AU - Xu, Wangjie

AU - Dai, Jingbo

AU - Chen, Xiaohui

AU - Zhao, Xianglong

AU - Fang, Peng

AU - Yang, Fang

AU - Tang, Maoping

AU - Wang, Zhaoxia

AU - Wang, Lianyun

AU - Ma, Duan

AU - Qaio, Zhongdong

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (No. 30901490 ), Medical Engineering in a joint project with Shanghai Jiao Tong University ( YG2010 MS27 ) and the National Key Basic Research Program ( 2009CB941704 ).

PY - 2013/3

Y1 - 2013/3

N2 - Smoking is associated with a declining quality of semen. The aim of this study was to screen and investigate the differential expression of proteins extracted from the epididymis of mice exposed daily with cigarette smoke. Using MALDI-TOF-MS analysis, we found that the protein profile of the mouse epididymis was altered by cigarette smoking and identified 27 proteins from the most abundant and differentially expressed spots in the 2-DE gels of epididymal samples. These proteins were classified into groups according to their functions such as energy metabolism, reproduction and structural molecule activity. Through pathway analysis, these proteins were associated with the glutathione metabolism and protein processing in the endoplasmic reticulum. These results showed that the epididymis may experience oxidative stress following cigarette smoke exposure, which was confirmed using immunohistochemistry. We determine that cigarette smoking can induce oxidative stress in the mouse epididymis, which may cause protein profile altering, thereby impairing epididymis function, and leading to a decline in semen quality.

AB - Smoking is associated with a declining quality of semen. The aim of this study was to screen and investigate the differential expression of proteins extracted from the epididymis of mice exposed daily with cigarette smoke. Using MALDI-TOF-MS analysis, we found that the protein profile of the mouse epididymis was altered by cigarette smoking and identified 27 proteins from the most abundant and differentially expressed spots in the 2-DE gels of epididymal samples. These proteins were classified into groups according to their functions such as energy metabolism, reproduction and structural molecule activity. Through pathway analysis, these proteins were associated with the glutathione metabolism and protein processing in the endoplasmic reticulum. These results showed that the epididymis may experience oxidative stress following cigarette smoke exposure, which was confirmed using immunohistochemistry. We determine that cigarette smoking can induce oxidative stress in the mouse epididymis, which may cause protein profile altering, thereby impairing epididymis function, and leading to a decline in semen quality.

KW - 2-D Gel electrophoresis

KW - Cigarette smoking

KW - Epididymis

KW - Male fertility

KW - Mass spectrometry

UR - http://www.scopus.com/inward/record.url?scp=84873329601&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84873329601&partnerID=8YFLogxK

U2 - 10.1016/j.biocel.2012.12.007

DO - 10.1016/j.biocel.2012.12.007

M3 - Article

C2 - 23262294

AN - SCOPUS:84873329601

SN - 1357-2725

VL - 45

SP - 571

EP - 582

JO - International Journal of Biochemistry and Cell Biology

JF - International Journal of Biochemistry and Cell Biology

IS - 3

ER -

The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this