The antigenicity of synthetic peptide fragments of lactate dehydrogenase C4

Thomas E. Wheat; Joyce A. Shelton; Victoria Gonzales-Prevatt; Erwin Goldberg

doi:10.1016/0161-5890(85)90008-2

The antigenicity of synthetic peptide fragments of lactate dehydrogenase C₄

Thomas E. Wheat, Joyce A. Shelton, Victoria Gonzales-Prevatt, Erwin Goldberg^*

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Lactate dehydrogenase C₄ (LDH-C₄) is an antigenic protein found only in spermatozoa and the mature testis. Synthetic peptides containing the amino acid sequences of the C-subunit, designated MC_5-15, MC_97-110 and MC_211-220, were each conjugated to the carrier proteins diphtheria toxoid or bovine serum albumin. Rabbits immunized with these peptide-carrier conjugates produced antibodies to the peptide that cross-reacted with native LDH-C₄. These data support our map that identifies antigenic domains of LDH-C₄. Such synthetic peptides will be useful in the design of a contraceptive vaccine.

Original language	English (US)
Pages (from-to)	1195-1199
Number of pages	5
Journal	Molecular Immunology
Volume	22
Issue number	10
DOIs	https://doi.org/10.1016/0161-5890(85)90008-2
State	Published - Oct 1985

ASJC Scopus subject areas

Molecular Biology
Immunology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/0161-5890(85)90008-2

Cite this

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title = "The antigenicity of synthetic peptide fragments of lactate dehydrogenase C4",

abstract = "Lactate dehydrogenase C4 (LDH-C4) is an antigenic protein found only in spermatozoa and the mature testis. Synthetic peptides containing the amino acid sequences of the C-subunit, designated MC5-15, MC97-110 and MC211-220, were each conjugated to the carrier proteins diphtheria toxoid or bovine serum albumin. Rabbits immunized with these peptide-carrier conjugates produced antibodies to the peptide that cross-reacted with native LDH-C4. These data support our map that identifies antigenic domains of LDH-C4. Such synthetic peptides will be useful in the design of a contraceptive vaccine.",

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T1 - The antigenicity of synthetic peptide fragments of lactate dehydrogenase C4

AU - Wheat, Thomas E.

AU - Shelton, Joyce A.

AU - Gonzales-Prevatt, Victoria

AU - Goldberg, Erwin

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N2 - Lactate dehydrogenase C4 (LDH-C4) is an antigenic protein found only in spermatozoa and the mature testis. Synthetic peptides containing the amino acid sequences of the C-subunit, designated MC5-15, MC97-110 and MC211-220, were each conjugated to the carrier proteins diphtheria toxoid or bovine serum albumin. Rabbits immunized with these peptide-carrier conjugates produced antibodies to the peptide that cross-reacted with native LDH-C4. These data support our map that identifies antigenic domains of LDH-C4. Such synthetic peptides will be useful in the design of a contraceptive vaccine.

AB - Lactate dehydrogenase C4 (LDH-C4) is an antigenic protein found only in spermatozoa and the mature testis. Synthetic peptides containing the amino acid sequences of the C-subunit, designated MC5-15, MC97-110 and MC211-220, were each conjugated to the carrier proteins diphtheria toxoid or bovine serum albumin. Rabbits immunized with these peptide-carrier conjugates produced antibodies to the peptide that cross-reacted with native LDH-C4. These data support our map that identifies antigenic domains of LDH-C4. Such synthetic peptides will be useful in the design of a contraceptive vaccine.

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