The AP-2 Complex Is Excluded from the Dynamic Population of Plasma Membrane-associated Clathrin

Joshua Z. Rappoport, Bushra W. Taha, Simone Lemeer, Alexandre Benmerah, Sanford M. Simon*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

55 Scopus citations


Numerous biologically relevant substrates are selectively internalized via clathrin-mediated endocytosis. At the plasma membrane the AP-2 complex plays a major role in clathrin coat formation, interacting with both cargo and clathrin. Utilizing simultaneous dual-channel total internal reflection fluorescence microscopy we have analyzed components of the AP-2 complex (α- and β2-adaptin) during clathrin-mediated endocytosis. Although in static images enhanced green fluorescent protein-tagged AP-2 markers significantly co-localized with clathrin and other components of clathrin-coated pits, AP-2 did not seem to be present in clathrin spots that appeared to undergo internalization or motility parallel to the plane of the plasma membrane. Two populations of clathrin at the plasma membrane seem to exist, the dynamic and the static, and AP-2 appears to be only found within the latter. These results suggest that co-localized clathrin/AP-2 puncta may represent loci for coated pit production and that previous models that assumed AP-2 was retained within clathrin coats during endocytosis may need to be re-evaluated.

Original languageEnglish (US)
Pages (from-to)47357-47360
Number of pages4
JournalJournal of Biological Chemistry
Issue number48
StatePublished - Nov 28 2003

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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