The Aspergillus nidulans cysA gene encodes a novel type of serine O-acetyltransferase which is homologous to homoserine O-acetyltransferases

M. Grynberg, J. Topczewski, A. Godzik, A. Paszewski*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The Aspergillus nidulans cysA gene was cloned by functional complementation of the cysA1 mutation that impairs the synthesis of O-acetylserine. The molecular nature of cysA1 and cysA103 alleles was characterized; a nucleotide substitution and a frame shift were found in the former and a deletion mutation in the latter. The CYSA protein is 525 amino acids long and is encoded by an uninterrupted open reading frame. Expression of the cysA gene appears not to be regulated by sulfur, carbon and nitrogen sources. Protein sequence analysis reveals extensive similarity to homoserine O-acetyltransferases, particularly the bacterial ones, and no homology with known serine O-acetyltransferases. The authors propose that the CYSA protein is analogous to serine O-acetyltransferases, i.e. it catalyses the same reaction but has an independent evolutionary origin.

Original languageEnglish (US)
Pages (from-to)2695-2703
Number of pages9
JournalMicrobiology
Volume146
Issue number10
DOIs
StatePublished - 2000

Keywords

  • A.nidulans
  • Analogous genes
  • Cysteine synthesis

ASJC Scopus subject areas

  • Microbiology

Fingerprint Dive into the research topics of 'The Aspergillus nidulans cysA gene encodes a novel type of serine O-acetyltransferase which is homologous to homoserine O-acetyltransferases'. Together they form a unique fingerprint.

Cite this