The avian retroviral IN protein is both necessary and sufficient for integrative recombination in vitro

Richard A. Katz*, George Merkel, Joseph Kulkosky, Jonathan Leis, Anna Marie Skalka

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

291 Scopus citations

Abstract

The integration of viral DNA into the host cell chromosome is an essential feature of the retroviral life cycle. The integration reaction requires cis-acting sequences at the end of linear viral DNA and a trans-acting product of the pol gene, the integration protein (IN). Previously, we demonstrated that avian sarcoma-leukosis virus (ASLV) IN is able to carry out the first step in the integration process in vitro: nicking of the ends of linear viral DNA. In this paper, using two independent assays, we demonstrate that IN, alone, is sufficient to carry out the second step: cleavage and joining to the target DNA. These results demonstrate that the retroviral IN protein is an integrase.

Original languageEnglish (US)
Pages (from-to)87-95
Number of pages9
JournalCell
Volume63
Issue number1
DOIs
StatePublished - Oct 5 1990

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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