The C. elegans gene lin-9, which acts in an Rb-related pathway, is required for gonadal sheath cell development and encodes a novel protein

Greg J. Beitel; Eric J. Lambie; H. Robert Horvitz

doi:10.1016/S0378-1119(00)00296-1

The C. elegans gene lin-9, which acts in an Rb-related pathway, is required for gonadal sheath cell development and encodes a novel protein

Greg J. Beitel, Eric J. Lambie, H. Robert Horvitz^*

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

The Caenorhabditis elegans gene lin-9 functions in an Rb-related pathway that acts antagonistically to a receptor tyrosine kinase/Ras signal transduction pathway controlling vulval induction. We show that lin-9 is also required for the development of the sheath cells in the hermaphrodite gonad and for the development of the male spicule, rays and gonad. lin-9 is transcribed as two alternatively spliced 2.4 kb messages, which use two distinct polyadenylation sites and are SL1 trans-spliced. The conceptual translation of lin-9 cDNA sequences predicts proteins of 642 and 644 amino acids with a significant similarity to predicted Drosophila and vertebrate proteins. We suggest that lin-9 is the founding member of a new protein family that functions in Rb-related pathways in many species. (C) 2000 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	253-263
Number of pages	11
Journal	Gene
Volume	254
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-1119(00)00296-1
State	Published - Aug 22 2000

Funding

We thank Jeffrey Thomas, Shai Shaham, Michael Koelle and Sander van den Heuvel for critical comments concerning this manuscript. We also thank current and past members of the Horvitz and Meyer laboratories for their helpful discussions and assistance including Jeffrey Thomas, Craig Ceol, Mark Metzstein, Michel Labouesse, Laird Bloom, Gian Garriga, and Leslie Lobel. We thank David Greenstein for the anti-CEH-18 antisera, and Helen White-Cooper and Minx Fuller for communicating unpublished data. Many appropriate references have not been cited because of limitations on the number of references allowed. This work was supported by United States Public Health Service grants GM24663 to H.R.H. and GM49785 to E.J.L. G.J.B. was a Predoctoral Fellow of the Howard Hughes Medical Institute. H.R.H. is an Investigator of the Howard Hughes Medical Institute.

Keywords

Alternative splicing
Ras
Rb Cell fate
Signal transduction
Vulval induction

ASJC Scopus subject areas

Genetics

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10.1016/S0378-1119(00)00296-1

Cite this

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title = "The C. elegans gene lin-9, which acts in an Rb-related pathway, is required for gonadal sheath cell development and encodes a novel protein",

abstract = "The Caenorhabditis elegans gene lin-9 functions in an Rb-related pathway that acts antagonistically to a receptor tyrosine kinase/Ras signal transduction pathway controlling vulval induction. We show that lin-9 is also required for the development of the sheath cells in the hermaphrodite gonad and for the development of the male spicule, rays and gonad. lin-9 is transcribed as two alternatively spliced 2.4 kb messages, which use two distinct polyadenylation sites and are SL1 trans-spliced. The conceptual translation of lin-9 cDNA sequences predicts proteins of 642 and 644 amino acids with a significant similarity to predicted Drosophila and vertebrate proteins. We suggest that lin-9 is the founding member of a new protein family that functions in Rb-related pathways in many species. (C) 2000 Elsevier Science B.V.",

keywords = "Alternative splicing, Ras, Rb Cell fate, Signal transduction, Vulval induction",

author = "Beitel, {Greg J.} and Lambie, {Eric J.} and Horvitz, {H. Robert}",

note = "Funding Information: We thank Jeffrey Thomas, Shai Shaham, Michael Koelle and Sander van den Heuvel for critical comments concerning this manuscript. We also thank current and past members of the Horvitz and Meyer laboratories for their helpful discussions and assistance including Jeffrey Thomas, Craig Ceol, Mark Metzstein, Michel Labouesse, Laird Bloom, Gian Garriga, and Leslie Lobel. We thank David Greenstein for the anti-CEH-18 antisera, and Helen White-Cooper and Minx Fuller for communicating unpublished data. Many appropriate references have not been cited because of limitations on the number of references allowed. This work was supported by United States Public Health Service grants GM24663 to H.R.H. and GM49785 to E.J.L. G.J.B. was a Predoctoral Fellow of the Howard Hughes Medical Institute. H.R.H. is an Investigator of the Howard Hughes Medical Institute.",

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N2 - The Caenorhabditis elegans gene lin-9 functions in an Rb-related pathway that acts antagonistically to a receptor tyrosine kinase/Ras signal transduction pathway controlling vulval induction. We show that lin-9 is also required for the development of the sheath cells in the hermaphrodite gonad and for the development of the male spicule, rays and gonad. lin-9 is transcribed as two alternatively spliced 2.4 kb messages, which use two distinct polyadenylation sites and are SL1 trans-spliced. The conceptual translation of lin-9 cDNA sequences predicts proteins of 642 and 644 amino acids with a significant similarity to predicted Drosophila and vertebrate proteins. We suggest that lin-9 is the founding member of a new protein family that functions in Rb-related pathways in many species. (C) 2000 Elsevier Science B.V.

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KW - Alternative splicing

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KW - Rb Cell fate

KW - Signal transduction

KW - Vulval induction

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The C. elegans gene lin-9, which acts in an Rb-related pathway, is required for gonadal sheath cell development and encodes a novel protein

Abstract

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Keywords

ASJC Scopus subject areas

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