Abstract
Mutations in the unc-104 gene of the nematode C. elegans result in uncoordinated and slow movement. Transposon insertions in three unc-104 alleles (e2184, rh 1016, and rh1017) were used as physical markers to clone the unc-104 gene. DNA sequence analysis of unc-104 cDNAs revealed an open reading frame capable of encoding a 1584 amino acid protein with similarities to kinesin heavy chain. The similarities are greatest in the aminoterminal ATPase and microtubule-binding domains. Although the primary sequence relatedness to kinesin is weak in the remainder of the molecule, the predicted secondary structure and regional isoelectric points are similar to kinesin heavy chain.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 113-122 |
| Number of pages | 10 |
| Journal | Neuron |
| Volume | 6 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1991 |
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ASJC Scopus subject areas
- Neuroscience(all)
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The C. elegans unc-104 4 gene encodes a putative kinesin heavy chain-like protein. / Otsuka, Anthony J.; Jeyaprakash, Ayyamperumal; García-Añoveros, Jaime; Tang, Lan Zhao; Fisk, Gregory; Hartshorne, Toinette; Franco, Rodrigo; Bornt, Teresa.
In: Neuron, Vol. 6, No. 1, 01.01.1991, p. 113-122.Research output: Contribution to journal › Article
TY - JOUR
T1 - The C. elegans unc-104 4 gene encodes a putative kinesin heavy chain-like protein
AU - Otsuka, Anthony J.
AU - Jeyaprakash, Ayyamperumal
AU - García-Añoveros, Jaime
AU - Tang, Lan Zhao
AU - Fisk, Gregory
AU - Hartshorne, Toinette
AU - Franco, Rodrigo
AU - Bornt, Teresa
PY - 1991/1/1
Y1 - 1991/1/1
N2 - Mutations in the unc-104 gene of the nematode C. elegans result in uncoordinated and slow movement. Transposon insertions in three unc-104 alleles (e2184, rh 1016, and rh1017) were used as physical markers to clone the unc-104 gene. DNA sequence analysis of unc-104 cDNAs revealed an open reading frame capable of encoding a 1584 amino acid protein with similarities to kinesin heavy chain. The similarities are greatest in the aminoterminal ATPase and microtubule-binding domains. Although the primary sequence relatedness to kinesin is weak in the remainder of the molecule, the predicted secondary structure and regional isoelectric points are similar to kinesin heavy chain.
AB - Mutations in the unc-104 gene of the nematode C. elegans result in uncoordinated and slow movement. Transposon insertions in three unc-104 alleles (e2184, rh 1016, and rh1017) were used as physical markers to clone the unc-104 gene. DNA sequence analysis of unc-104 cDNAs revealed an open reading frame capable of encoding a 1584 amino acid protein with similarities to kinesin heavy chain. The similarities are greatest in the aminoterminal ATPase and microtubule-binding domains. Although the primary sequence relatedness to kinesin is weak in the remainder of the molecule, the predicted secondary structure and regional isoelectric points are similar to kinesin heavy chain.
UR - http://www.scopus.com/inward/record.url?scp=0025968679&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025968679&partnerID=8YFLogxK
U2 - 10.1016/0896-6273(91)90126-K
DO - 10.1016/0896-6273(91)90126-K
M3 - Article
VL - 6
SP - 113
EP - 122
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 1
ER -