The carcinogen benzo(e)pyrene is metabolized by DM15 cells without an uncoupling effect on their gap junctions

Irina V. Budunova; Leonid A. Mittleman; Radjab D. Safaev; Serge Yu Fuchs; Gennady A. Belitsky

doi:10.1007/BF00757575

The carcinogen benzo(e)pyrene is metabolized by DM15 cells without an uncoupling effect on their gap junctions

Irina V. Budunova^*, Leonid A. Mittleman, Radjab D. Safaev, Serge Yu Fuchs, Gennady A. Belitsky

^*Corresponding author for this work

Dermatology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Benzo(e)pyrene (B(e)P) promotes carcinogenesis in the skin. Unlike some other promoters however, B(e)P does notproduce an uncoupling effect on gap junction permeability in DM15 transformedfibroblasts. This study demonstrates thatDM15 cells exhibit a relatively high level of B(e)P metabolism. Moreover, although pretreatment of DM15 cells with benz(a)anthracene results in an 8-fold increase of arylhydrocarbon hydroxylase activity and a 2-fold increase in the rate ofB(e)P metabolism, it did not enable B(e)P to affectLucifer Yellow transfer between DM15 cells. We conclude that neitherB(e)P nor its metabolites are capable of uncoupling gap junction permeability in DM15 cells.

Original language	English (US)
Pages (from-to)	131-140
Number of pages	10
Journal	Cell Biology and Toxicology
Volume	9
Issue number	2
DOIs	https://doi.org/10.1007/BF00757575
State	Published - Jun 1 1993

Keywords

benzo(e)pyrene dye transfer
intercellular communication

ASJC Scopus subject areas

Toxicology
Cell Biology
Health, Toxicology and Mutagenesis

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10.1007/BF00757575

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title = "The carcinogen benzo(e)pyrene is metabolized by DM15 cells without an uncoupling effect on their gap junctions",

abstract = "Benzo(e)pyrene (B(e)P) promotes carcinogenesis in the skin. Unlike some other promoters however, B(e)P does notproduce an uncoupling effect on gap junction permeability in DM15 transformedfibroblasts. This study demonstrates thatDM15 cells exhibit a relatively high level of B(e)P metabolism. Moreover, although pretreatment of DM15 cells with benz(a)anthracene results in an 8-fold increase of arylhydrocarbon hydroxylase activity and a 2-fold increase in the rate ofB(e)P metabolism, it did not enable B(e)P to affectLucifer Yellow transfer between DM15 cells. We conclude that neitherB(e)P nor its metabolites are capable of uncoupling gap junction permeability in DM15 cells.",

keywords = "benzo(e)pyrene dye transfer, intercellular communication",

author = "Budunova, {Irina V.} and Mittleman, {Leonid A.} and Safaev, {Radjab D.} and Fuchs, {Serge Yu} and Belitsky, {Gennady A.}",

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AU - Budunova, Irina V.

AU - Mittleman, Leonid A.

AU - Safaev, Radjab D.

AU - Fuchs, Serge Yu

AU - Belitsky, Gennady A.

PY - 1993/6/1

Y1 - 1993/6/1

N2 - Benzo(e)pyrene (B(e)P) promotes carcinogenesis in the skin. Unlike some other promoters however, B(e)P does notproduce an uncoupling effect on gap junction permeability in DM15 transformedfibroblasts. This study demonstrates thatDM15 cells exhibit a relatively high level of B(e)P metabolism. Moreover, although pretreatment of DM15 cells with benz(a)anthracene results in an 8-fold increase of arylhydrocarbon hydroxylase activity and a 2-fold increase in the rate ofB(e)P metabolism, it did not enable B(e)P to affectLucifer Yellow transfer between DM15 cells. We conclude that neitherB(e)P nor its metabolites are capable of uncoupling gap junction permeability in DM15 cells.

AB - Benzo(e)pyrene (B(e)P) promotes carcinogenesis in the skin. Unlike some other promoters however, B(e)P does notproduce an uncoupling effect on gap junction permeability in DM15 transformedfibroblasts. This study demonstrates thatDM15 cells exhibit a relatively high level of B(e)P metabolism. Moreover, although pretreatment of DM15 cells with benz(a)anthracene results in an 8-fold increase of arylhydrocarbon hydroxylase activity and a 2-fold increase in the rate ofB(e)P metabolism, it did not enable B(e)P to affectLucifer Yellow transfer between DM15 cells. We conclude that neitherB(e)P nor its metabolites are capable of uncoupling gap junction permeability in DM15 cells.

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The carcinogen benzo(e)pyrene is metabolized by DM15 cells without an uncoupling effect on their gap junctions

Abstract

Keywords

ASJC Scopus subject areas

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