TY - JOUR
T1 - The Caspase-3 homolog DrICE regulates endocytic trafficking during Drosophila tracheal morphogenesis
AU - McSharry, Saoirse S.
AU - Beitel, Greg J.
N1 - Funding Information:
For reagents we thank Rob Ward (α-Uif), Pascal Meier (α-DrICESK31), Christos Sama-kovlis (α-MTf), and Matthias Behr (α-Clathrin). We also thank Benjamin Kraft for assistance in epistasis experiments; Andreas Bergmann for helpful discussions; J. Hornick and the Northwestern University Biological Imaging Facility for technical and imaging support; and Laura Lackner, Heike Fölsch, and I. Tanelli Helenius for comments on the manuscript. Stocks obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537) were used in this study, and FlyBase was a critical resource. This work was supported by NIH RO1GM108964 to G.J.B. S.S.M. was supported by the National Institute of General Medical Sciences training grant T32GM008061.
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Although well known for its role in apoptosis, the executioner caspase DrICE has a non-apoptotic function that is required for elongation of the epithelial tubes of the Drosophila tracheal system. Here, we show that DrICE acts downstream of the Hippo Network to regulate endocytic trafficking of at least four cell polarity, cell junction and apical extracellular matrix proteins involved in tracheal tube size control: Crumbs, Uninflatable, Kune-Kune and Serpentine. We further show that tracheal cells are competent to undergo apoptosis, even though developmentally-regulated DrICE function rarely kills tracheal cells. Our results reveal a developmental role for caspases, a pool of DrICE that co-localizes with Clathrin, and a mechanism by which the Hippo Network controls endocytic trafficking. Given reports of in vitro regulation of endocytosis by mammalian caspases during apoptosis, we propose that caspase-mediated regulation of endocytic trafficking is an evolutionarily conserved function of caspases that can be deployed during morphogenesis.
AB - Although well known for its role in apoptosis, the executioner caspase DrICE has a non-apoptotic function that is required for elongation of the epithelial tubes of the Drosophila tracheal system. Here, we show that DrICE acts downstream of the Hippo Network to regulate endocytic trafficking of at least four cell polarity, cell junction and apical extracellular matrix proteins involved in tracheal tube size control: Crumbs, Uninflatable, Kune-Kune and Serpentine. We further show that tracheal cells are competent to undergo apoptosis, even though developmentally-regulated DrICE function rarely kills tracheal cells. Our results reveal a developmental role for caspases, a pool of DrICE that co-localizes with Clathrin, and a mechanism by which the Hippo Network controls endocytic trafficking. Given reports of in vitro regulation of endocytosis by mammalian caspases during apoptosis, we propose that caspase-mediated regulation of endocytic trafficking is an evolutionarily conserved function of caspases that can be deployed during morphogenesis.
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U2 - 10.1038/s41467-019-09009-z
DO - 10.1038/s41467-019-09009-z
M3 - Article
C2 - 30833576
AN - SCOPUS:85062397219
VL - 10
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 1031
ER -