The chick embryo appears as a natural model for research in beta-amyloid precursor protein processing

J. A. Carrodeguas, A. Rodolosse, M. V. Garza, A. Sanz-Clemente, R. Pérez-Pé, A. M. Lacosta, L. Domínguez, I. Monleón, R. Sánchez-Díaz, V. Sorribas, M. Sarasa*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

This study reveals that the chick embryo has active the machinery for the production and degradation of the amyloid beta peptide characteristic of Alzheimer's disease. We cloned the principal beta-amyloid precursor protein isoforms in the chick embryo and observed that they are highly homologous to the human sequences and identical at the C-terminal sequence, including the amyloid beta domain. Mammals such as rat or mouse, more commonly used as animal models of human diseases, have a distinct amyloid beta sequence. The distribution of beta-amyloid precursor protein isoforms in the chick embryo revealed that, as in humans, their expression is ubiquitous and the prototype beta-amyloid precursor protein-695 predominated in the nervous system. We also found that the chick embryo expresses the genes for the main proteolytic proteases implicated in the production of amyloid beta, including BACE-1, BACE-2, presenilin-1, presenilin-2 and nicastrin, as well as the amyloid beta-degrading enzyme neprilysin, or ADAM-17, a protease implicated in the non-amyloidogenic processing of beta-amyloid precursor protein. We have also found that between amyloid beta40 and amyloid beta42, this latter seems to be the major amyloid beta peptide produced during chick embryogenesis. The chick embryo appears as a suitable natural model to study cell biology and developmental function of beta-amyloid precursor protein and a potential assay system for drugs that regulate beta-amyloid precursor protein processing.

Original languageEnglish (US)
Pages (from-to)1285-1300
Number of pages16
JournalNeuroscience
Volume134
Issue number4
DOIs
StatePublished - 2005

Keywords

  • ADAM
  • APP
  • Alzheimer's disease
  • Chick embryo
  • Neprilysin
  • Secretase

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'The chick embryo appears as a natural model for research in beta-amyloid precursor protein processing'. Together they form a unique fingerprint.

Cite this